CRISP1 Human
Cysteine-Rich Secretory Protein 1 Human Recombinant
Cat. No.
BT26116
Source
Escherichia Coli.
Synonyms
Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
The CRISP1 Protein Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (Lys 22 - Lys 249) having a molecular mass of 27kDa.
CRISP1 is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
CRISP1 is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
The process of insemination involves a complex series of cell-to-cell interactions that culminate in the fusion of the sperm and egg cell membranes. This intricate process relies on the precise recognition, binding, and fusion of complementary molecules located on specific regions of both the sperm and egg cell surfaces. In sperm, the region known as the postacrosomal region, or equatorial segment, plays a crucial role in the fusion with the egg's membrane. The protein encoded by the CRISP1 gene is a key player in this process. CRISP1 belongs to a family of proteins known as cysteine-rich secretory proteins (CRISPs), which are involved in various reproductive processes. Specifically, CRISP1 is produced in the epididymis, a coiled tube located in the testes, and is secreted into the epididymal lumen, the fluid-filled space within the epididymis where sperm mature. Once in the lumen, CRISP1 binds to the postacrosomal region of the sperm head, where it is strategically positioned to participate in the critical sperm-egg fusion event.
Description
CRISP1 Protein, Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 238 amino acids (Lys 22 - Lys 249). It has a molecular weight of 27 kDa. For purification and detection purposes, a 10 amino acid His-tag is fused to the N-terminus of the protein. The protein undergoes purification using proprietary chromatographic techniques.
Purity
Purity of CRISP1 Protein is greater than 85% as determined by SDS-PAGE analysis.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a solution containing 20mM TRIS, 50mM NaCl, at pH 7.5, with a protein concentration of 0.5mg/ml.
Solubility
To reconstitute lyophilized CRISP1, it is recommended to dissolve the protein in sterile 18MΩ-cm H₂O to a concentration of at least 100µg/ml. This solution can then be further diluted to desired concentrations using other aqueous solutions.
Stability
Lyophilized CRISP1 protein remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized protein in a desiccated state below -18°C. Once reconstituted, CRISP1 should be stored at 4°C for a period of 2-7 days. For extended storage after reconstitution, it is recommended to store the protein below -18°C. It is crucial to avoid repeated freeze-thaw cycles to maintain protein integrity and activity.
Applications
This product is suitable for use in various research applications, including Western blotting and ELISA.
Synonyms
Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS KKKSARDQFN KLVTDLPNVQ EEIVNIHNAL RRRVVPPASN MLKMSWSEEA AQNARIFSKY CDMTESNPLE RRLPNTFCGE NMHMTSYPVS WSSVIGVWYS ESTSFKHGEW TTTDDDITTD HYTQIVWATS YLIGCAIASC RQQGSPRYLY VCHYCHEGND PETKNEPYKT GVPCEACPSN CEDKLCTNPC IYYDEYFDCD IQVHYLGCNH STTILFCKAT CLCDTEIK
Product Science Overview
Introduction
Cysteine-Rich Secretory Protein 1 (CRISP1) is a member of the cysteine-rich secretory protein (CRISP) family, which belongs to the larger CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1) superfamily. These proteins are characterized by their high cysteine content and are known for their diverse roles in various biological processes, including reproduction, immune defense, and ion channel regulation .
Structure and Function
CRISP1, like other members of the CRISP family, contains a conserved CAP domain and a cysteine-rich domain. The CAP domain is involved in the regulation of extracellular matrix and branching morphogenesis, while the cysteine-rich domain is associated with ion channel regulation . CRISP1 is primarily expressed in the male reproductive tract, particularly in the epididymis, where it plays a crucial role in sperm maturation and fertilization .
Biological Roles
- Reproduction: CRISP1 is essential for the proper functioning of the male reproductive system. It is involved in the maturation of spermatozoa and their ability to fertilize an egg. Studies have shown that CRISP1 interacts with the zona pellucida of the egg, facilitating sperm-egg fusion .
- Immune Defense: Members of the CRISP family, including CRISP1, have been implicated in immune defense mechanisms. They are known to modulate the immune response and protect against pathogens .
- Ion Channel Regulation: CRISP1 has been shown to regulate ion channels, which are critical for various physiological processes. This regulation is essential for maintaining cellular homeostasis and proper functioning of tissues .
Recombinant CRISP1
Recombinant CRISP1 is produced using recombinant DNA technology, which involves inserting the gene encoding CRISP1 into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of CRISP1 for research and therapeutic purposes. Recombinant CRISP1 retains the structural and functional properties of the native protein, making it a valuable tool for studying its biological roles and potential applications in medicine .
Applications in Research and Medicine
- Fertility Treatments: Given its role in sperm maturation and fertilization, recombinant CRISP1 has potential applications in fertility treatments. It can be used to enhance sperm function and improve the success rates of assisted reproductive technologies .
- Immune Modulation: The immune-modulatory properties of CRISP1 make it a potential candidate for developing therapies for immune-related disorders. Recombinant CRISP1 can be used to study its effects on the immune system and develop novel treatments .
- Ion Channel Disorders: By regulating ion channels, CRISP1 can be targeted for the treatment of ion channel-related disorders. Recombinant CRISP1 can be used to investigate its role in these disorders and develop therapeutic interventions .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
