CoV-2 Spike S1 (200-800)
CoV 2019 Spike S1 Protein is >90% pure as determined SDS-PAGE.
Description
The E.Coli derived recombinant protein contains the Coronavirus 2019 Spike S1 (200-800 a.a.) region, fused to 6xHis tag at C-terminal
Product Specs
In December 2019, a novel coronavirus, designated as 2019-nCoV, emerged in Wuhan, China, causing viral pneumonia in humans. The virus was first identified in connection with a seafood market.
Genetic analysis revealed that 2019-nCoV shares a high degree of similarity (87%) with a bat-derived SARS-like coronavirus (SARS-CoV-2) discovered in Zhoushan, eastern China, in 2018. Despite some genetic variations, the receptor-binding domain (RBD) of 2019-nCoV is structurally similar to that of SARS-CoV, suggesting its potential to bind to the human ACE2 (angiotensin-converting enzyme 2) receptor.
While bats are considered the likely natural reservoir of 2019-nCoV, the intermediary animal responsible for its transmission to humans remains unknown, although seafood sold at the Wuhan market was suspected. Research suggests that 2019-nCoV may be a recombinant virus, with its spike glycoprotein showing evidence of genetic material from both bat coronaviruses and an unidentified coronavirus.
This recombinant protein, expressed in E. coli, encompasses the S1 subunit (amino acids 200-800) of the SARS-CoV-2 Spike protein. It features a C-terminal 6xHis tag for purification and detection purposes.
This product consists of a 1 mg/ml solution of the CoV 2019 Spike S1 (200-800 a.a.) Protein. It is formulated in 1x PBS (phosphate-buffered saline).
The CoV 2019 Spike S1 (200-800 a.a.) Protein is shipped with ice packs to maintain its stability. Upon receipt, it should be stored at -20°C (-4°F).
The purity of the CoV 2019 Spike S1 Protein is greater than 90%, as determined by SDS-PAGE analysis.
Product Science Overview
The Coronavirus 2019 Spike S1 (200-800 a.a.) Recombinant is a crucial component in the study and understanding of the SARS-CoV-2 virus, which causes COVID-19. This recombinant protein is a segment of the spike protein, specifically the S1 subunit, which plays a vital role in the virus’s ability to infect host cells.
The spike protein of SARS-CoV-2 is a transmembrane protein that protrudes from the viral surface, giving the virus its characteristic crown-like appearance. The spike protein is divided into two subunits: S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for binding to the host cell receptor, angiotensin-converting enzyme 2 (ACE2). This binding is the first step in the viral entry process.
The recombinant form of the spike protein, specifically the S1 subunit (200-800 amino acids), is produced using recombinant DNA technology. This involves inserting the gene encoding the spike protein into an expression system, such as bacteria or mammalian cells, to produce the protein in large quantities.
The recombinant spike S1 protein is used in various research and diagnostic applications:
- Vaccine Development: The spike protein is a primary target for vaccine development because it is the main antigen that elicits an immune response. Recombinant spike proteins are used to develop subunit vaccines, which contain only the antigenic parts of the virus.
- Diagnostic Assays: The recombinant spike S1 protein is used in serological assays to detect antibodies against SARS-CoV-2 in patient samples. These assays help determine whether an individual has been exposed to the virus and has developed an immune response.
- Therapeutic Research: Researchers use the recombinant spike protein to study the interaction between the virus and the host cell receptor. This research is crucial for developing therapeutic agents that can block viral entry and prevent infection.
The production of recombinant spike S1 protein involves several steps:
- Gene Cloning: The gene encoding the spike protein is cloned into an expression vector, which is then introduced into an expression system such as E. coli or HEK293 cells.
- Protein Expression: The expression system produces the spike protein, which is then harvested from the cells.
- Purification: The recombinant protein is purified using techniques such as affinity chromatography, which isolates the protein based on its specific binding properties.
