CoV-2-S1
HEK293
Protein is >85% pure as determined SDS-PAGE.
Description
The HEK293 derived recombinant protein contains the Novel Coronavirus 2019-nCoV Spike Glycoprotein S1, Wuhan-Hu-1 strain, amino acids 1-674 fused to Fc tag at C-terminal.
Product Specs
In December 2019, a novel coronavirus, designated as 2019-nCoV, emerged in Wuhan, China, causing viral pneumonia in humans. The virus was initially linked to a seafood market.
Genetic analysis revealed that 2019-nCoV shares a high degree of similarity (87%) with a bat-derived SARS-like coronavirus (SARS-CoV-2) discovered in Zhoushan, China. Despite some genetic variations, the receptor-binding domain (RBD) of 2019-nCoV closely resembles that of SARS-CoV, suggesting a potential to bind to the human ACE2 receptor.
While bats are suspected to be the natural reservoir of 2019-nCoV, an intermediate animal host, possibly traded at the Wuhan market, is thought to have played a role in its transmission to humans. Research indicates that 2019-nCoV might have arisen from a recombination event involving a bat coronavirus and another unknown coronavirus, particularly in the spike glycoprotein region.
This recombinant protein consists of the Spike Glycoprotein S1 subunit (amino acids 1-674) of the 2019 Novel Coronavirus (2019-nCoV), specifically the Wuhan-Hu-1 strain. It is produced in HEK293 cells and is fused with an Fc tag at its C-terminus.
The nCoV-S1 protein solution is provided in a solution of Dulbecco's Phosphate-Buffered Saline (DPBS) at a pH of 7.4.
To maintain product integrity, the protein is shipped with ice packs to ensure a cool temperature during transport. Upon receiving the protein, it should be stored at a temperature of -20 degrees Celsius.
The purity of the protein is greater than 85%, as determined by SDS-PAGE analysis.
HEK293
Purified by Protein-G chromatographic technique.
Product Science Overview
The Coronavirus 2019 Spike Glycoprotein-S1 (SARS-CoV-2 Spike S1) is a crucial component of the virus responsible for the COVID-19 pandemic. This glycoprotein plays a pivotal role in the virus’s ability to infect host cells. The recombinant form of this protein is widely used in research and vaccine development.
The Spike (S) glycoprotein of SARS-CoV-2 is a trimeric protein that protrudes from the viral surface. It is divided into two subunits: S1 and S2. The S1 subunit contains the receptor-binding domain (RBD), which is responsible for binding to the host cell receptor, angiotensin-converting enzyme 2 (ACE2). This binding is the first step in the viral entry process .
Recombinant SARS-CoV-2 Spike S1 proteins are produced using various expression systems, including HEK293 cells and CHO cells. These systems allow for the production of high-purity proteins suitable for research applications. The recombinant proteins are often tagged with polyhistidine (His) tags to facilitate purification .
- Vaccine Development: The recombinant Spike S1 protein is used in the development of vaccines. It serves as an antigen to elicit an immune response in the host, leading to the production of neutralizing antibodies.
- Diagnostic Tools: The protein is used in various diagnostic assays, including ELISA, to detect antibodies against SARS-CoV-2 in patient samples.
- Therapeutic Research: Researchers use the recombinant protein to study the interaction between the virus and host cells, which can lead to the development of antiviral drugs .
