SARS Spike (14-1195)
Sf9, Baculovirus cells.
Spike glycoprotein, S glycoprotein, Peplomer protein, E2 glycoprotein precursor, Severe acute repiratory Syndrome-related Coronavirus, SARS, SRAS-CoV, SARS-CoV1, E2.
Greater than 85.0% as determined by SDS-PAGE.
Description
SARS Spike produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 1188 amino acids (14-1195 aa) and having a molecular mass of 131.9kDa.
SARS Spike is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
This section provides a brief overview of the SARS Coronavirus, highlighting its structure and the significance of the Spike (S) protein in viral entry and as a target for neutralizing antibodies.
This part details the characteristics of the SARS Spike protein produced in Sf9 Baculovirus cells, including its amino acid length, molecular weight, glycosylation status, His tag, and purification method.
This section specifies the components and concentration of the SARS Spike (14-1195) solution, which includes Phosphate-Buffered Saline (pH 7.4) and Glycerol.
This part provides instructions for storing the product, including recommended temperatures and the addition of carrier proteins for long-term storage. It also advises avoiding repeated freezing and thawing.
This indicates the purity level of the SARS Spike protein, determined by SDS-PAGE analysis, to be higher than 85%.
This section describes how the biological activity of the SARS Spike protein is assessed, which is by measuring its binding affinity to Human ACE-2 using a functional ELISA.
Spike glycoprotein, S glycoprotein, Peplomer protein, E2 glycoprotein precursor, Severe acute repiratory Syndrome-related Coronavirus, SARS, SRAS-CoV, SARS-CoV1, E2.
Sf9, Baculovirus cells.
SDLDRCTTFD DVQAPNYTQH TSSMRGVYYP DEIFRSDTLY LTQDLFLPFY SNVTGFHTIN HTFGNPVIPF KDGIYFAATE KSNVVRGWVF GSTMNNKSQS VIIINNSTNV VIRACNFELC DNPFFAVSKP MGTQTHTMIF DNAFNCTFEY ISDAFSLDVS EKSGNFKHLR EFVFKNKDGF LYVYKGYQPI DVVRDLPSGF NTLKPIFKLP LGINITNFRA ILTAFSPAQD IWGTSAAAYF VGYLKPTTFM LKYDENGTIT DAVDCSQNPL AELKCSVKSF EIDKGIYQTS NFRVVPSGDV VRFPNITNLC PFGEVFNATK FPSVYAWERK KISNCVADYS VLYNSTFFST FKCYGVSATK LNDLCFSNVY ADSFVVKGDD VRQIAPGQTG VIADYNYKLP DDFMGCVLAW NTRNIDATST GNYNYKYRYL RHGKLRPFER DISNVPFSPD GKPCTPPALN CYWPLNDYGF YTTTGIGYQP YRVVVLSFEL LNAPATVCGP KLSTDLIKNQ CVNFNFNGLT GTGVLTPSSK RFQPFQQFGR DVSDFTDSVR DPKTSEILDI SPCAFGGVSV ITPGTNASSE VAVLYQDVNC TDVSTAIHAD QLTPAWRIYS TGNNVFQTQA GCLIGAEHVD TSYECDIPIG AGICASYHTV SLLRSTSQKS IVAYTMSLGA DSSIAYSNNT IAIPTNFSIS ITTEVMPVSM AKTSVDCNMY ICGDSTECAN LLLQYGSFCT QLNRALSGIA AEQDRNTREV FAQVKQMYKT PTLKYFGGFN FSQILPDPLK PTKRSFIEDL LFNKVTLADA GFMKQYGECL GDINARDLIC AQKFNGLTVL PPLLTDDMIA AYTAALVSGT ATAGWTFGAG AALQIPFAMQ MAYRFNGIGV TQNVLYENQK QIANQFNKAI SQIQESLTTT STALGKLQDV VNQNAQALNT LVKQLSSNFG AISSVLNDIL SRLDKVEAEV QIDRLITGRL QSLQTYVTQQ LIRAAEIRAS ANLAATKMSE CVLGQSKRVD FCGKGYHLMS FPQAAPHGVV FLHVTYVPSQ ERNFTTAPAI CHEGKAYFPR EGVFVFNGTS WFITQRNFFS PQIITTDNTF VSGNCDVVIG IINNTVYDPL QPELDSFKEE LDKYFKNHTS PDVDLGDISG INASVVNIQK EIDRLNEVAK NLNESLIDLQ ELGKYEQYIK WPHHHHHH
Product Science Overview
The SARS-associated coronavirus spike protein, specifically the segment spanning amino acids 14 to 1195, plays a crucial role in the virus’s ability to infect host cells. This recombinant protein is a key target for research and vaccine development due to its involvement in the virus’s entry mechanism.
The spike protein of SARS-CoV (Severe Acute Respiratory Syndrome Coronavirus) is a transmembrane glycoprotein that forms homotrimers protruding from the viral surface. It is responsible for binding to the host cell receptor, angiotensin-converting enzyme 2 (ACE2), and facilitating viral entry through membrane fusion. The spike protein is composed of two subunits:
- S1 subunit: Contains the receptor-binding domain (RBD) that directly interacts with ACE2.
- S2 subunit: Mediates the fusion of the viral and host cell membranes.
The segment from amino acids 14 to 1195 encompasses the majority of the spike protein, including the entire S1 subunit and a significant portion of the S2 subunit.
Recombinant production of the spike protein involves cloning the gene encoding the desired segment into an expression vector, which is then introduced into a host cell system (such as bacteria, yeast, or mammalian cells). The host cells produce the spike protein, which can be purified for use in various applications, including:
- Vaccine development: The spike protein is a primary antigen used in vaccine formulations to elicit an immune response.
- Diagnostic assays: Recombinant spike protein can be used in serological tests to detect antibodies against SARS-CoV.
- Structural studies: High-resolution structures of the spike protein, obtained through techniques like cryo-electron microscopy, provide insights into its function and interactions with host receptors.
The spike protein’s role in viral entry makes it a critical focus for research aimed at understanding the mechanisms of SARS-CoV infection and developing therapeutic interventions. Studies have shown that mutations in the spike protein can affect its binding affinity to ACE2 and influence the virus’s transmissibility and pathogenicity .
