CFB (260-764) Human, Sf9
CFB, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, BF, BFD, AHUS4, ARMD14, CFAB, CFBD, FB, FBI12, GBG, H2-Bf.
Greater than 90.0% as determined by SDS-PAGE.
Description
CFB Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 514 amino acids (260-764 a.a) and having a molecular mass of 58.1kDa.
CFB is fused to an 9 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
CFB, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, BF, BFD, AHUS4, ARMD14, CFAB, CFBD, FB, FBI12, GBG, H2-Bf.
ADPKIVLDPS GSMNIYLVLD GSDSIGASNF TGAKKCLVNL IEKVASYGVK PRYGLVTYAT
YPKIWVKVSE ADSSNADWVT KQLNEINYED HKLKSGTNTK KALQAVYSMM SWPDDVPPEG
WNRTRHVIIL MTDGLHNMGG DPITVIDEIR DLLYIGKDRK NPREDYLDVY VFGVGPLVNQ
VNINALASKK DNEQHVFKVK DMENLEDVFY QMIDESQSLS LCGMVWEHRK GTDYHKQPWQ
AKISVIRPSK GHESCMGAVV SEYFVLTAAH CFTVDDKEHS IKVSVGGEKR DLEIEVVLFH
PNYNINGKKE AGIPEFYDYD VALIKLKNKL KYGQTIRPIC LPCTEGTTRA LRLPPTTTCQ
QQKEELLPAQ DIKALFVSEE EKKLTRKEVY IKNGDKKGSC ERDAQYAPGY DKVKDISEVV
TPRFLCTGGV SPYADPNTCR GDSGGPLIVH KRSRFIQVGV ISWGVVDVCK NQKRQKQVPA HARDFHINLF QVLPWLKEKL QDEDLGFLHH HHHH
Product Science Overview
Complement Factor B is a single-chain glycoprotein that circulates in the blood. It is cleaved by complement factor D into two fragments: Ba and Bb. The Bb fragment is a serine protease that combines with complement factor 3b (C3b) to form the C3 or C5 convertase, which is essential for the activation of the complement pathway . The Ba fragment acts as an inhibitor for the proliferation of lymphocytes .
The recombinant form of Complement Factor B (260-764 a.a.) is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain containing 514 amino acids (260-764 a.a.) and has a molecular mass of approximately 58.1 kDa . The protein is fused to a 9 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques .
Recombinant Complement Factor B is used in various research applications, including studies on the complement system, immune response, and related diseases. It is also utilized in biochemical assays to understand the mechanisms of complement activation and regulation.
The recombinant protein is typically supplied as a sterile, filtered colorless solution. It is formulated in phosphate-buffered saline (PBS) with 20% glycerol to enhance stability. For short-term storage, it is recommended to keep the protein at 4°C if it will be used within 2-4 weeks. For long-term storage, it should be frozen at -20°C, and it is advisable to add a carrier protein (0.1% HSA or BSA) to prevent degradation .
