CFB Human, Sf9

Complement Factor B is a single-chain polypeptide with a molecular mass of approximately 93 kDa . Upon activation of the alternative pathway, it is cleaved by complement factor D into two fragments: the non-catalytic chain Ba (30 kDa) and the catalytic subunit Bb (63 kDa) . The Bb subunit, a serine protease, associates with C3b to form the alternative pathway C3 convertase (C3bBb), which is essential for the amplification of the complement response .

The recombinant form of Complement Factor B is produced using Sf9 Baculovirus cells. This method involves the expression of the CFB gene in insect cells, resulting in a glycosylated polypeptide chain containing 514 amino acids and having a molecular mass of 58.1 kDa . The recombinant protein is fused to a 9-amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques .

Recombinant Complement Factor B is used primarily for laboratory research purposes. It is supplied as a sterile, filtered, colorless solution formulated with phosphate-buffered saline (pH 7.4) and 20% glycerol . The protein should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and avoid multiple freeze-thaw cycles to maintain stability .

The study of Complement Factor B is vital for understanding the mechanisms of the alternative complement pathway and its role in immune responses. Research on recombinant forms of this protein helps in elucidating its structure-function relationships and potential therapeutic applications in immune-related disorders.

By leveraging recombinant technology, scientists can produce high-quality Complement Factor B for various experimental applications, contributing to advancements in immunology and therapeutic development.