CFB Human
Complement Factor B Human Recombinant
Cat. No.
BT18105
Source
Escherichia Coli.
Synonyms
Complement factor B (EC:3.4.21.47), C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, Complement Factor B, BFD, AHUS4, BF, BFD, CFAB, FB, FBI12, H2-Bf.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CFB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 528 amino acids (260-764) and having a molecular mass of 59.4 kDa.
CFB is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
CFB is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Complement Factor B, also known as CFB, is a protein involved in the complement system, a part of the immune system. It helps activate the alternative pathway of complement activation. This pathway is important for destroying pathogens (harmful microorganisms) and removing damaged cells from the body. When activated, CFB is cleaved into two parts: Ba and Bb. Bb is the active part and works with another protein, C3b, to form a complex that helps break down pathogens.
Description
This product is a laboratory-made version of the human CFB protein. It is produced in bacteria (E.coli) and purified to a high degree. It is provided as a solution with a concentration of 1 mg/ml. This recombinant protein is a single chain of 528 amino acids with a molecular weight of 59.4 kDa. It contains a His-tag at the N-terminus for easy purification.
Physical Appearance
Clear and colorless solution that has been sterilized by filtration.
Formulation
This solution contains 1 mg of CFB per ml, 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the solution refrigerated at 4°C. For longer storage, freeze the solution at -20°C. To ensure stability during long-term storage, it is advised to add a carrier protein like HSA or BSA (0.1%). Avoid repeatedly freezing and thawing the solution.
Purity
The purity of this product is over 85%, as determined by SDS-PAGE, a method used to separate and analyze proteins.
Synonyms
Complement factor B (EC:3.4.21.47), C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, Complement Factor B, BFD, AHUS4, BF, BFD, CFAB, FB, FBI12, H2-Bf.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKIVLDPS GSMNIYLVLD GSDSIGASNF TGAKKCLVNL IEKVASYGVK PRYGLVTYAT YPKIWVKVSE ADSSNADWVT KQLNEINYED HKLKSGTNTK KALQAVYSMM SWPDDVPPEG WNRTRHVIIL MTDGLHNMGG DPITVIDEIR DLLYIGKDRK NPREDYLDVY VFGVGPLVNQ VNINALASKK DNEQHVFKVK DMENLEDVFY QMIDESQSLS LCGMVWEHRK GTDYHKQPWQ AKISVIRPSK GHESCMGAVV SEYFVLTAAH CFTVDDKEHS IKVSVGGEKR DLEIEVVLFH PNYNINGKKE AGIPEFYDYD VALIKLKNKL KYGQTIRPIC LPCTEGTTRA LRLPPTTTCQ QQKEELLPAQ DIKALFVSEE EKKLTRKEVY IKNGDKKGSC ERDAQYAPGY DKVKDISEVV TPRFLCTGGV SPYADPNTCR GDSGGPLIVH KRSRFIQVGV ISWGVVDVCK NQKRQKQVPA HARDFHINLF QVLPWLKEKL QDEDLGFL.
Product Science Overview
Structure and Function
Complement Factor B circulates in the blood as a single-chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D into two fragments: Ba and Bb . The Ba fragment is non-catalytic, while the Bb fragment is a serine protease that associates with C3b to form the alternative pathway C3 convertase . This convertase is essential for the amplification of the complement response, leading to the opsonization of pathogens, recruitment of inflammatory cells, and lysis of target cells.
Genetic and Molecular Characteristics
Recombinant Production
Recombinant human Complement Factor B is produced using various expression systems, including HEK293 cells and Escherichia coli . The recombinant protein is often tagged with a polyhistidine tag (His tag) to facilitate purification. The production process involves the following steps:
- Gene Cloning: The gene encoding Complement Factor B is cloned into an expression vector.
- Transformation: The vector is introduced into the host cells (e.g., HEK293 or E. coli).
- Expression: The host cells are cultured under conditions that promote the expression of the recombinant protein.
- Purification: The recombinant protein is purified using affinity chromatography, exploiting the His tag for binding to a nickel or cobalt resin.
- Validation: The purity and activity of the recombinant protein are validated using techniques such as SDS-PAGE and functional assays.
Applications
Recombinant Complement Factor B is used in various research applications, including:
- Studying the Complement System: Researchers use recombinant Complement Factor B to investigate the mechanisms of the alternative pathway and its role in immune responses.
- Drug Development: It is used in the development of therapeutic agents targeting the complement system for diseases such as age-related macular degeneration and atypical hemolytic uremic syndrome.
- Diagnostic Assays: Recombinant Complement Factor B is employed in diagnostic assays to measure complement activity and identify deficiencies or dysregulations in the complement pathway.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
