C5a Protein
Human Plasma.
Complement Component 5, C3 and PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 4, C5a Anaphylatoxin, Prepro-C5, CPAMD4, Anaphylatoxin C5a Analog, ECLZB, C5A, C5D, C5b, C5.
Sterile filtered solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
Human Complement C5a produced in Human plasma having a molecular mass of 10.4 kDa.
Product Specs
Complement C5a (C5a) is a protein involved in the complement system, a crucial part of the body's immune response. C5a is created when a specific protein, C5, is split into two parts: C5a and C5b. C5a acts like a signal flare, attracting immune cells to the site of infection or damage. This process is called chemotaxis. Meanwhile, C5b helps form a complex that can punch holes in the membranes of invading cells.
This product contains human Complement C5a, a protein with a molecular weight of 10.4 kDa. It is produced using human plasma.
The product is a clear, sterile solution that has been passed through a filter to remove any bacteria.
The C5a protein is supplied in a solution containing 120 mM NaCl (sodium chloride) and 10mM HEPES (a buffering agent) at a pH of 7.2.
To ensure product stability, store C5a Human at 4°C and use within 2-4 weeks. For longer-term storage, freeze at or below -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeatedly freezing and thawing the product.
This product has a purity greater than 95%, as determined by SDS-PAGE, a technique used to separate and analyze proteins.
The plasma used to produce this product has been rigorously tested and confirmed negative for a range of viruses, including HIV-1, HIV-2, HCV, HTLV-I & II, STS, and HBSAG, ensuring product safety.
Complement Component 5, C3 and PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 4, C5a Anaphylatoxin, Prepro-C5, CPAMD4, Anaphylatoxin C5a Analog, ECLZB, C5A, C5D, C5b, C5.
Human Plasma.
Product Science Overview
Human C5a is a 74 amino acid peptide with a molecular weight of approximately 11 kDa . The structure of C5a consists of four helices connected by peptide loops, stabilized by three disulfide bonds. The N-terminus contains a short helix, but the primary agonist activity is located at the C-terminus .
C5a is known for its role as an anaphylatoxin and a chemotactic agent . It has several key functions:
- Anaphylatoxin Activity: C5a causes increased expression of adhesion molecules on endothelial cells, contraction of smooth muscle, and increased vascular permeability. It can also trigger mast cell degranulation, leading to the release of proinflammatory molecules such as histamine and TNF-α .
- Chemotaxis: C5a is a potent chemoattractant, initiating the accumulation of complement and phagocytic cells at sites of infection. It also recruits antigen-presenting cells to lymph nodes .
- Immune Cell Activation: C5a enhances the migration and adherence of neutrophils and monocytes to vessel walls. It activates white blood cells by upregulating integrin avidity and modulating the balance between activating and inhibitory IgG Fc receptors on leukocytes .
The increased production of C5a is associated with various inflammatory diseases. Abnormal complement activation, including the generation of C5a, can lead to conditions such as paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome . Therapeutic interventions targeting C5a and its receptor (C5aR) are being explored to treat these and other inflammatory diseases .
Recombinant human C5a is produced using various expression systems, including bacterial, yeast, and mammalian cells. These recombinant proteins are used in research and therapeutic applications to study the functions of C5a and to develop potential treatments for diseases involving complement activation .
