C5a Mouse

Complement Component C5a is a 77 amino acid peptide generated from the cleavage of the C5 alpha-chain by the enzyme C5a convertase . This cleavage occurs in both the classical and alternative complement pathways, resulting in the formation of C5a and C5b. The recombinant version of mouse C5a is typically produced in E. coli and purified to high levels of purity (>97%) for research purposes .

C5a is known as an anaphylatoxin, a type of molecule that can induce rapid degranulation of mast cells and basophils, leading to the release of histamine and other inflammatory mediators . It is a potent chemoattractant, recruiting immune cells such as neutrophils, eosinophils, and macrophages to sites of infection or injury . Additionally, C5a enhances the phagocytic activity of these cells, aiding in the clearance of pathogens .

C5a exerts its effects by binding to its specific receptor, C5aR (CD88), which is expressed on the surface of various immune cells . This binding triggers a cascade of intracellular signaling events that result in the activation of these cells and the release of inflammatory cytokines and chemokines . The interaction between C5a and C5aR is crucial for the amplification of the inflammatory response and the recruitment of immune cells to the site of infection .

Recombinant mouse C5a is widely used in research to study the complement system and its role in various diseases. It is used in in vitro assays to investigate the effects of C5a on immune cell activation, chemotaxis, and cytokine production . Additionally, it is employed in animal models to study the role of C5a in inflammatory diseases, such as sepsis, arthritis, and autoimmune disorders .