C-JUN Human (241 a.a.)

The Jun Proto-Oncogene, also known as c-Jun, is a crucial component of the Activator Protein 1 (AP-1) transcription factor complex. This protein plays a significant role in regulating gene expression in response to a variety of stimuli, including stress, cytokines, growth factors, and infections.

The human recombinant Jun Proto-Oncogene (1-241 a.a.) is a non-glycosylated polypeptide chain consisting of 261 amino acids, including a 20 amino acid His tag at the N-terminus . This recombinant protein is produced in Escherichia coli (E. coli) and has a molecular mass of approximately 27.3 kDa .

c-Jun is highly similar to the viral protein and interacts directly with specific target DNA sequences to regulate gene expression . It is activated by the c-Jun N-terminal kinase (JNK) pathway and forms a part of the AP-1 early response transcription factor in combination with c-Fos . The c-Jun gene is intronless and is mapped to the chromosomal region 1p32-p31, which is involved in both translocations and deletions in human malignancies .

The recombinant form of c-Jun is widely used in laboratory research to study gene expression regulation, signal transduction pathways, and the molecular mechanisms underlying various diseases, including cancer . It is also utilized in drug development and screening processes.

The c-Jun recombinant protein is typically stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to keep it frozen at -20°C with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity.