C-JUN Human
Description
C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The Jun proto-oncogene, also known as c-Jun, is a crucial component of the AP-1 transcription factor complex. This gene plays a significant role in regulating gene expression in response to various stimuli, including stress, cytokines, growth factors, and infections. The human recombinant form of this protein is often used in research to study its functions and interactions.
The Jun proto-oncogene is located on chromosome 1p32-p31 in humans . It encodes a protein that is highly similar to the viral oncoprotein v-jun, discovered in avian sarcoma virus 17 . The human JUN gene is intronless, meaning it does not contain introns within its coding sequence .
The protein encoded by the JUN gene is a part of the AP-1 transcription factor complex, which also includes proteins like c-Fos. This complex binds to specific DNA sequences to regulate the transcription of various genes .
The primary function of the Jun protein is to regulate gene expression by binding to the AP-1 binding site on DNA. This regulation is crucial for various cellular processes, including cell proliferation, differentiation, and apoptosis .
Jun and its dimerization partners in the AP-1 complex are regulated by diverse extracellular stimuli, such as peptide growth factors, pro-inflammatory cytokines, oxidative stress, and UV irradiation . For instance, UV irradiation is a potent inducer of elevated c-Jun expression . The transcription of c-Jun can be autoregulated by its own product, creating a positive feedback loop that prolongs the signals from extracellular stimuli .
The Jun proto-oncogene is classified as a proto-oncogene because its deregulation can lead to cancer. It was the first oncogenic transcription factor discovered . The protein’s activity is regulated by the ERK pathway, and constitutively active ERK can increase c-Jun transcription and stability . This regulation is significant for the activity of c-Jun in cancer, as it can lead to the activation of downstream targets like RACK1 and cyclin D1, which are involved in cell cycle progression and tumorigenesis .
Human recombinant Jun protein is widely used in research to study its role in gene regulation and its implications in cancer. By using the recombinant form, researchers can investigate the protein’s interactions, regulatory mechanisms, and potential as a therapeutic target.
