Chlamydia W3-W6

Chlamidia Trachomatis W3-W6 Recombinant
Cat. No.
BT797
Source
Synonyms
Appearance
Purity
Chlamydia W3-W6 protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

The E.coli derived recombinant 6xHis fusion at C-terminus. protein contains Chlamydia TrachomatisMOMP protein epitopes, 128-398 amino acids.

Product Specs

Introduction
Chlamydia is a common term for infections caused by bacteria from the phylum Chlamydiae. The name originates from the Chlamydia genus within the Chlamydiaceae family, belonging to the Chlamydiales order, and ultimately the Chlamydiae class and phylum. The Chlamydiaceae family includes two genera: Chlamydia and Chlamydophila. Three species fall under the Chlamydia genus: C. trachomatis, C. muridarum, and C. suis.
Description
This recombinant protein is derived from E. coli and contains a 6xHis fusion tag at the C-terminus. It encompasses amino acids 128-398 of the Chlamydia Trachomatis MOMP protein.
Purity
The purity of the Chlamydia W3-W6 protein exceeds 95%, as determined by 10% PAGE (Coomassie staining) and RP-HPLC analysis.
Formulation
The protein is supplied in a buffer composed of 10mM Tris-HCl (pH 6.5), 100mM Sodium Phosphate, and 8M urea.
Stability
Chlamydia W3-W6 protein remains stable at 4°C for up to one week. For long-term storage, it is recommended to store the protein below -18°C. Avoid repeated freeze-thaw cycles.
Applications
Chlamydia W3-W6 is suitable for use in ELISA. The optimal working titer for specific applications should be determined by each laboratory. While other applications have not been tested, their use should not be ruled out.
Purification Method
Chlamydia W3-W6 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of Chlamydia Trachomatis infected individuals.

Product Science Overview

Genetic Variation and Recombination

Genetic variation is crucial for the long-term survival of bacteria. In the case of Chlamydia trachomatis, mutation and recombination are the fundamental processes by which genetic variation emerges. Recombination involves the incorporation of imported DNA into the genome, leading to the introduction of novel sequences and the creation of loci with mosaic genes. This process plays a significant role in the potential for adaptation of a bacterial population .

Recombinant Chlamydia Trachomatis W3-W6

The W3-W6 recombinant protein of Chlamydia trachomatis is a specific segment of the bacterium’s protein structure. This recombinant protein is typically produced using Escherichia coli as a host for expression. The W3-W6 protein is purified using proprietary chromatographic techniques to achieve a purity level of over 95%, as determined by PAGE (Polyacrylamide Gel Electrophoresis) and RP-HPLC (Reverse Phase High-Performance Liquid Chromatography) .

Applications and Specificity

The recombinant W3-W6 protein is immunoreactive with sera from individuals infected with Chlamydia trachomatis. This makes it highly valuable for use in immunization protocols and generating antibodies that specifically target the protein of interest. The high sensitivity, specificity, and reproducibility of this recombinant protein make it ideal for various research and diagnostic applications .

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