Chlamydia HSP70 (549-660a.a.)

Heat shock proteins (HSPs) are a group of proteins that are produced by cells in response to stressful conditions. They play a crucial role in protein folding, repair, and protection against cellular stress. Among these, HSP70 is one of the most studied due to its significant role in cellular homeostasis and stress response .

The HSP70 protein of Chlamydia trachomatis is particularly interesting because it is synthesized during infection and released into the bloodstream. This protein can elicit an immune response, leading to the production of anti-HSP70 antibodies . The specific segment of HSP70 from amino acids 549 to 660 is often studied for its immunogenic properties.

Recombinant proteins are artificially synthesized proteins that are produced through recombinant DNA technology. The recombinant Chlamydia trachomatis HSP70 (549-660 a.a.) is produced in E. coli and is used for various research purposes. This recombinant protein contains epitopes from the HSP70 protein, specifically from amino acids 549 to 660 .

Formulation and Purity:

• The recombinant protein is formulated in 50 mM Tris-HCl (pH 8.0), 60 mM NaCl, 8 M Urea, and 50% glycerol .
• It is highly pure, with a purity level of over 95% as determined by 10% PAGE (Coomassie staining) and RP-HPLC .

Applications:

• The recombinant HSP70 protein is primarily used in ELISA (Enzyme-Linked Immunosorbent Assay) to detect antibodies against Chlamydia trachomatis in infected individuals .
• It is also used in various research applications to study the immune response to Chlamydia trachomatis and to develop potential diagnostic tools and vaccines .

Storage and Stability:

• The protein is stable at 4°C for one week but should be stored below -18°C for long-term storage to prevent freeze-thaw cycles .