Chlamydia HSP70 (462-503 a.a.)
Description
Product Specs
Product Science Overview
Chlamydia trachomatis is a bacterium responsible for various infections in humans, including trachoma, which can lead to blindness, and sexually transmitted infections. One of the key proteins associated with Chlamydia trachomatis is the Heat Shock Protein 70 (HSP70). This protein plays a crucial role in the bacterium’s survival and pathogenicity.
Heat Shock Proteins (HSPs) are a group of proteins that are produced by cells in response to stressful conditions. HSP70, in particular, is involved in protein folding, protection of cells from stress, and aiding in the recovery of cells from damage. In the context of Chlamydia trachomatis, HSP70 is essential for the bacterium’s ability to infect host cells and evade the host’s immune response.
The recombinant form of Chlamydia trachomatis HSP70 (462-503 amino acids) is a specific segment of the HSP70 protein. This segment is produced using recombinant DNA technology, which involves inserting the gene encoding this protein segment into a host organism, such as E. coli, to produce the protein in large quantities.
The preparation of recombinant Chlamydia trachomatis HSP70 (462-503 a.a.) involves several steps:
- Gene Cloning: The gene encoding the HSP70 segment (462-503 a.a.) is cloned into a suitable expression vector.
- Transformation: The expression vector is introduced into a host organism, commonly E. coli.
- Protein Expression: The host organism is cultured under conditions that induce the expression of the recombinant protein.
- Protein Purification: The expressed protein is purified using techniques such as affinity chromatography to obtain a high-purity product.
The recombinant Chlamydia trachomatis HSP70 (462-503 a.a.) protein is used in various research applications, including:
- Vaccine Development: Studying the immune response to this protein segment can aid in the development of vaccines against Chlamydia trachomatis infections.
- Diagnostic Tools: The protein can be used to develop diagnostic assays for detecting Chlamydia trachomatis infections.
- Research: Understanding the role of HSP70 in the pathogenesis of Chlamydia trachomatis can provide insights into potential therapeutic targets.
