CEA Human, His
Carcinoembryonic Antigen Related Cell Adhesion Molecule 5, Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5, Meconium Antigen 100, CEA, Carcinoembryonic Antigen, CD66e Antigen, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA.
Greater than 95.0% as determined by SDS-PAGE.
Description
CEA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 659 amino acids (35-685a.a.) and having a molecular mass of 72.3kDa (Molecular size on SDS-PAGE will appear at approximately 70-150kDa). CEA is expressed with an 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Carcinoembryonic Antigen Related Cell Adhesion Molecule 5, Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5, Meconium Antigen 100, CEA, Carcinoembryonic Antigen, CD66e Antigen, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA.
KLTIESTPFN VAEGKEVLLL VHNLPQHLFG YSWYKGERVD GNRQIIGYVI GTQQATPGPA YSGREIIYPN ASLLIQNIIQ NDTGFYTLHV IKSDLVNEEA TGQFRVYPEL PKPSISSNNS KPVEDKDAVA FTCEPETQDA TYLWWVNNQS LPVSPRLQLS NGNRTLTLFN VTRNDTASYK CETQNPVSAR RSDSVILNVL YGPDAPTISP LNTSYRSGEN LNLSCHAASN PPAQYSWFVN GTFQQSTQEL FIPNITVNNS GSYTCQAHNS DTGLNRTTVT TITVYAEPPK PFITSNNSNP VEDEDAVALT CEPEIQNTTY LWWVNNQSLP VSPRLQLSND NRTLTLLSVT RNDVGPYECG IQNKLSVDHS DPVILNVLYG PDDPTISPSY TYYRPGVNLS LSCHAASNPP AQYSWLIDGN IQQHTQELFI SNITEKNSGL YTCQANNSAS GHSRTTVKTI TVSAELPKPS ISSNNSKPVE DKDAVAFTCE PEAQNTTYLW WVNGQSLPVS PRLQLSNGNR TLTLFNVTRN DARAYVCGIQ NSVSANRSDP VTLDVLYGPD TPIISPPDSS YLSGANLNLS CHSASNPSPQ YSWRINGIPQ QHTQVLFIAK ITPNNNGTYA CFVSNLATGR NNSIVKSITV SASGTSPGLS ALEHHHHHH.
Product Science Overview
The Human Recombinant CEA with His Tag is produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain containing 659 amino acids and has a molecular mass of approximately 72.3 kDa . The recombinant protein is expressed with an 8 amino acid His tag at the C-terminus, which facilitates its purification through chromatographic techniques .
CEA is an important biomarker in oncology. Its levels are used to screen for cancer, evaluate the recurrence or dissemination of the disease, and determine the success of surgical removal of malignant tumors . Normal CEA levels range from 0.0 to 2.5 ng/ml in non-smokers, and levels above 20 ng/ml before treatment are often associated with metastatic cancer . Monitoring CEA levels post-treatment can help in detecting tumor recurrence .
The recombinant CEA protein with His tag is used in various research applications, including:
- ELISA (Enzyme-Linked Immunosorbent Assay): For detecting and quantifying CEA levels in biological samples.
- SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis): For analyzing the purity and molecular weight of the protein.
- Cell Adhesion Studies: To investigate the role of CEA in cell adhesion and signaling.
The recombinant CEA protein is supplied as a sterile, filtered colorless solution. It is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . For short-term storage, it can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with a carrier protein to prevent multiple freeze-thaw cycles .
