CD93 Human
CD93 Human Recombinant
Cat. No.
BT30878
Source
Sf9, Baculovirus cells.
Synonyms
CD93 Molecule, CD93 Antigen, Complement Component 1, Q Subcomponent, Receptor 1, Complement Component 1 Q Subcomponent Receptor 1, Matrix-Remodeling-Associated Protein 4, Matrix-Remodelling Associated 4, C1q/MBL/SPA Receptor, C1qR(P), C1Qrp, C1QR1, MXRA4, CDw93, C1qR, C1q Receptor 1, DJ737E23.1, ECSM3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CD93 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 567 amino acids (22-580 aa) and having a molecular mass of 59.3kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions).
CD93 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
CD93 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD93 is a cell surface receptor that plays multiple roles in immune defense and cellular adhesion. It serves as a receptor for complement components C1q and mannose-binding lectin (MBL2), as well as pulmonary surfactant protein A (SPA). This interaction enhances phagocytosis by monocytes and macrophages, particularly in the presence of soluble defense collagens. CD93 also contributes to intercellular adhesion. Notably, CD93 is expressed on plasma cells, including long-lived ones residing in bone marrow niches. These CD93-expressing plasma cells exhibit characteristics of maturity and functionality, such as reduced cell cycle activity, elevated antibody secretion, and specific transcriptional profiles. CD93 is crucial for the survival and maintenance of these plasma cell populations within the bone marrow.
Description
Recombinant human CD93 protein expressed in Sf9 insect cells using a baculovirus expression system. This protein is a single, glycosylated polypeptide chain comprising 567 amino acids (residues 22-580), with an 8-amino acid Histidine tag added at the C-terminus. The molecular weight is approximately 59.3 kDa, although it migrates at a higher apparent molecular weight (70-100 kDa) on SDS-PAGE under reducing conditions. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless liquid, sterile filtered.
Formulation
CD93 protein is supplied in a solution of Phosphate Buffered Saline (PBS), pH 7.4, containing 10% glycerol, at a concentration of 0.5 mg/ml.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure long-term stability during frozen storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided to prevent protein degradation.
Purity
The purity of CD93 protein is greater than 90.0% as assessed by SDS-PAGE analysis.
Synonyms
CD93 Molecule, CD93 Antigen, Complement Component 1, Q Subcomponent, Receptor 1, Complement Component 1 Q Subcomponent Receptor 1, Matrix-Remodeling-Associated Protein 4, Matrix-Remodelling Associated 4, C1q/MBL/SPA Receptor, C1qR(P), C1Qrp, C1QR1, MXRA4, CDw93, C1qR, C1q Receptor 1, DJ737E23.1, ECSM3.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
TGADTEAVVC VGTACYTAHS GKLSAAEAQN HCNQNGGNLA TVKSKEEAQH VQRVLAQLLR REAALTARMS KFWIGLQREK GKCLDPSLPL KGFSWVGGGE DTPYSNWHKE LRNSCISKRC VSLLLDLSQP LLPSRLPKWS EGPCGSPGSP GSNIEGFVCK FSFKGMCRPL ALGGPGQVTY TTPFQTTSSS LEAVPFASAA NVACGEGDKD ETQSHYFLCK EKAPDVFDWG SSGPLCVSPK YGCNFNNGGC HQDCFEGGDG SFLCGCRPGF RLLDDLVTCA SRNPCSSSPC RGGATCVLGP HGKNYTCRCP QGYQLDSSQL DCVDVDECQD SPCAQECVNT PGGFRCECWV GYEPGGPGEG ACQDVDECAL GRSPCAQGCT NTDGSFHCSC EEGYVLAGED GTQCQDVDEC VGPGGPLCDS LCFNTQGSFH CGCLPGWVLA PNGVSCTMGP VSLGPPSGPP DEEDKGEKEG STVPRAATAS PTRGPEGTPK ATPTTSRPSL SSDAPITSAP LKMLAPSGSP GVWREPSIHH ATAASGPQEP AGGDSSVATQ NNDGTDGQKV EHHHHHH
Product Science Overview
Structure and Composition
CD93 is a highly glycosylated protein with a molecular mass of approximately 120 kDa . The mature human CD93 consists of a 557 amino acid extracellular domain (ECD) that includes one C-type lectin domain, four tandem EGF-like domains, and a mucin-like domain . This is followed by a 21 amino acid transmembrane segment and a 51 amino acid cytoplasmic domain .
Expression and Function
CD93 is primarily expressed on endothelial cells, monocytes, neutrophils, and stem cells . It plays a crucial role in various physiological and pathological processes, including:
- Angiogenesis: CD93 is essential for promoting angiogenesis, the formation of new blood vessels, which is vital for both normal physiology and disease conditions .
- Phagocytosis: CD93 modulates the phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro .
- Inflammatory Reactions: CD93 is involved in various aspects of inflammatory reactions, contributing to the body’s immune response .
Recombinant Human CD93
Recombinant human CD93 is produced using DNA sequences encoding the human CD93 extracellular domain. This recombinant protein is typically expressed in HEK293 cells and is fused with a polyhistidine tag at the C-terminus . The secreted recombinant human CD93 comprises 570 amino acids and has a predicted molecular mass of 59.6 kDa. Due to glycosylation, it migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions .
Applications
Recombinant human CD93 is used in various research applications, including:
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