CD90 Thy-1 Antibody

Thy-1 (CD90), Rat Anti-Mouse
Cat. No.
BT4857
Source
Synonyms
CD-90, CD90, THY-1, THY1, Theta Antigen.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
CD-90, also known as Thy-1, is a surface glycoprotein involved in cell-cell and cell-ligand interactions, playing a crucial role in synaptogenesis and other brain functions. Found on thymocytes and neurons, Thy-1 is believed to be involved in cell-cell interactions. In humans, the Thy-1 gene maps to chromosome 11, similar to the T3D locus. In mice, both T3D and Thy-1 are located on chromosome 9, along with other loci homologous to human chromosome 11q.
Formulation
Provided as a 1 mg/ml solution in phosphate-buffered saline (PBS) after reconstitution.
Storage Procedures
Store the lyophilized antibody at 4°C. After reconstitution, the antibody is stable for one month at 4°C. For long-term storage, aliquot the reconstituted antibody and store at -20°C.
Solubility
Reconstitute the antibody by adding deionized water (H2O). Mix gently by inverting the vial several times. Rinse the vial sides with the reconstituted solution and allow 30-60 seconds for complete dissolution before use.
Applications
This antibody can be used for cytotoxic and staining applications. For staining purposes, use 10 µl of antibody per 1,000,000 cells. Notably, this antibody exhibits high efficiency in in vivo killing assays. The optimal cytotoxic titer should be determined empirically by the investigator.
Available Conjugates
This antibody is exclusively supplied in its purified form without any conjugates.
Synonyms
CD-90, CD90, THY-1, THY1, Theta Antigen.
Purification Method
Ion exchange column.
Type
Rat Anti Mouse Monoclonal.
Clone
mThy-1.
Immunogen
Purified mouse LN T cells.
Ig Subclass
Rat IgG1.

Product Science Overview

Introduction

Thy-1, also known as CD90, is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that belongs to the immunoglobulin superfamily. It was first identified as a T cell marker in 1964 by Reif and Allen during their search for heterologous antisera against mouse leukemia cells . Thy-1 is expressed on various cell types, including thymocytes, T lymphocytes, neurons, and hematopoietic stem cells .

Structure and Function

Thy-1 is a small protein, approximately 25-37 kDa in size, and is highly conserved across species. It plays a crucial role in cell-cell and cell-matrix interactions, signal transduction, and cellular adhesion. The protein is involved in various biological processes, including T cell activation, neurite outgrowth, apoptosis, and fibrosis .

Expression in Different Species
  • Rat: In rats, CD90 is expressed on thymocytes, recent thymic emigrants, hematopoietic stem cells, neurons, and other cell types .
  • Mouse: In mice, CD90.1 (Thy1.1) is expressed on thymocytes, hematopoietic stem cells in the bone marrow, intraepithelial cells in the skin, and neurons such as retinal ganglion cells . It is a pan T cell marker in mouse strains AKR/J, PL, and FVB/N .
Applications

The rat anti-mouse Thy-1 (CD90) antibody is widely used in research for various applications:

  • Cell Isolation: CD90.1 MicroBeads are used for the positive selection or depletion of mouse or rat T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or peripheral blood .
  • Flow Cytometry: The antibody is used for immunofluorescent staining and flow cytometric analysis to study the expression of CD90 on different cell types .
  • Immunohistochemistry: It is also used for staining acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections .

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