Introduction
CD-90, also known as Thy-1, is a cell surface glycoprotein implicated in cell-cell and cell-ligand interactions during crucial neurological processes like synaptogenesis. Primarily found on T cells, Thy-1 glycoproteins are also present on thymocytes and neurons, suggesting a role in cellular communication. The gene encoding human Thy-1 is located on chromosome 11, similar to the T3D locus. In mice, both T3D and Thy-1 map to chromosome 9, alongside other loci found on human chromosome 11q.
Formulation
Supplied as a lyophilized powder, this antibody can be reconstituted to a concentration of 1mg/ml using phosphate-buffered saline (PBS).
Storage Procedures
Store the lyophilized antibody at 4°C. Upon reconstitution, the antibody can be stored at 4°C for up to one month. For long-term storage, aliquot and store at -20°C.
Solubility
Reconstitute the lyophilized antibody by adding the appropriate volume of sterile H2O. Gently mix the solution by inverting the vial several times. Avoid shaking or vortexing. Allow the reconstituted antibody to sit at room temperature for 30-60 seconds before use.
Applications
This antibody is suitable for both cytotoxic and staining applications. For staining procedures, a recommended starting concentration is 10µl of antibody per 1,000,000 cells. The optimal titer for cytotoxicity assays should be determined empirically by the investigator.
Available Conjugates
This antibody is currently available only in its purified form.
Synonyms
CD-90, CD90, THY-1, THY1, Theta Antigen.
Purification Method
boric acid precipitation.
Type
Rat Anti Mouse Monoclonal.
Immunogen
Purified mouse LN T cells.