CD84 Human, Sf9
Sf9, Baculovirus cells.
CD84 Molecule, Signaling Lymphocytic Activation Molecule 5, Leukocyte Differentiation Antigen CD84, CD84 Antigen (Leukocyte Antigen), Cell Surface Antigen MAX.3, Hly9-Beta, SLAMF5, Leucocyte Differentiation Antigen CD84, Leukocyte Antigen CD84, SLAM Family Member 5, CD84 Antigen, HCD84, MCD84, LY9B, SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5.
Greater than 95% as determined by SDS-PAGE.
Description
CD84 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 213 amino acids (22-225 a.a.) and having a molecular mass of 23.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD84 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
As a member of the signaling lymphocyte activation molecule (SLAM) family, which is a subset of the larger CD2 cell-surface receptor Ig superfamily, CD84 plays a crucial role in immune system function. CD84 is a homophilic adhesion molecule that is expressed on a variety of immune cells, including T cells, B cells, and macrophages. CD84 helps to regulate receptor-mediated signaling in these cells, contributing to the modulation of immune responses.
Recombinant human CD84, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 213 amino acids (specifically, amino acids 22-225). It has a molecular mass of 23.8 kDa. However, it appears as a band with an approximate size of 28-40 kDa on SDS-PAGE analysis. The protein is engineered with a 6-amino acid His tag at the C-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
The provided CD84 protein solution has a concentration of 0.5 mg/ml. It is formulated in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
The purity of the CD84 protein is determined to be greater than 95% based on SDS-PAGE analysis.
CD84 Molecule, Signaling Lymphocytic Activation Molecule 5, Leukocyte Differentiation Antigen CD84, CD84 Antigen (Leukocyte Antigen), Cell Surface Antigen MAX.3, Hly9-Beta, SLAMF5, Leucocyte Differentiation Antigen CD84, Leukocyte Antigen CD84, SLAM Family Member 5, CD84 Antigen, HCD84, MCD84, LY9B, SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5.
Sf9, Baculovirus cells.
ADPKDSEIFT VNGILGESVT FPVNIQEPRQ VKIIAWTSKT SVAYVTPGDS ETAPVVTVTH RNYYERIHAL GPNYNLVISD LRMEDAGDYK ADINTQADPY TTTKRYNLQI YRRLGKPKIT QSLMASVNST CNVTLTCSVE KEEKNVTYNW SPLGEEGNVL QIFQTPEDQE LTYTCTAQNP
VSNNSDSISA RQLCADIAMG FRTHHTGHHH HHH.
Product Science Overview
The CD84 Human Recombinant protein produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 213 amino acids (22-225 a.a.) and has a molecular mass of 23.8 kDa. However, on SDS-PAGE, it appears at approximately 28-40 kDa due to glycosylation . The recombinant protein is expressed with a 6 amino acids His tag at the C-Terminus, which facilitates its purification through chromatographic techniques .
CD84 is involved in immune cell signaling and adhesion. It participates in the formation of the immunological synapse, a specialized junction between a T cell and an antigen-presenting cell, which is essential for effective immune responses. CD84’s role in immune regulation makes it a significant molecule for studying immune responses and potential therapeutic targets for immune-related diseases .
The recombinant CD84 protein is produced in Sf9 Baculovirus cells, a common system for producing recombinant proteins. This system allows for proper folding and post-translational modifications, such as glycosylation, which are essential for the protein’s functionality. The protein is purified using proprietary chromatographic techniques to achieve a purity greater than 95% as determined by SDS-PAGE .
Recombinant CD84 protein is used in various research applications, including:
- Studying immune cell interactions: Understanding how CD84 mediates cell-cell adhesion and signaling in the immune system.
- Drug development: Identifying potential therapeutic targets for immune-related diseases.
- Structural biology: Analyzing the structure-function relationship of CD84 and its interactions with other molecules.
The CD84 protein solution is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol. For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to prevent degradation. It is crucial to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
