CD84 Human
Description
CD84 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CD84 is a homophilic receptor, meaning it can bind to itself, and it plays a crucial role in the modulation of both adaptive and innate immune responses . The protein is composed of an extracellular domain, a transmembrane domain, and a cytoplasmic tail . The extracellular domain contains immunoglobulin (Ig) superfamily domains, specifically a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain, which are responsible for ligand recognition .
The cytoplasmic tail of CD84 contains immunoreceptor tyrosine-based switch motifs (ITSMs), which interact with adaptor molecules such as SLAM-associated protein (SAP) and EWS-activated transcript 2 (EAT-2) . These interactions are essential for signal transduction and the subsequent modulation of immune responses .
CD84 is predominantly expressed on the surface of various immune cells, including T cells, B cells, natural killer (NK) cells, and dendritic cells . Its expression is upregulated upon activation of these cells, indicating its role in immune cell signaling and activation .
The CD84-CD84 interaction is independent of its cytoplasmic tail, suggesting that the extracellular domain alone is sufficient for its homophilic binding and signaling functions . This interaction acts as a costimulatory signal, enhancing the activation and proliferation of immune cells .
Recombinant human CD84 is produced using various expression systems, including HEK293 cells and mouse myeloma cell lines (NS0) . The recombinant protein is typically tagged with a polyhistidine (His) tag to facilitate purification and detection . The purified protein is used in various research applications, including studies on immune cell signaling, receptor-ligand interactions, and the development of therapeutic antibodies .
Recombinant CD84 is available in different formulations, including those with and without carrier proteins such as bovine serum albumin (BSA) . The presence of carrier proteins can enhance the stability and shelf-life of the recombinant protein, making it suitable for various experimental applications .
CD84 is a valuable tool in immunological research due to its role in immune cell signaling and activation. Studies involving recombinant CD84 have provided insights into the mechanisms of immune cell interactions, the regulation of immune responses, and the potential therapeutic applications of targeting CD84 in immune-related diseases .
