CD68 Human, sf9

CD68 Human Recombinant, sf9
Cat. No.
BT30407
Source
Sf9, Baculovirus cells.
Synonyms
Macrosialin, CD68, Gp110, Macrosialin isoform A, GP110, LAMP4, SCARD1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD68 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 307 amino acids (22-319) and having a molecular mass of 32.6kDa (Molecular size on SDS-PAGE will appear at approximately 57-70kDa).
CD68 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD68 is a gene that provides instructions for producing a protein found in high concentrations on monocytes and tissue macrophages. This protein, also known as CD68, belongs to the LAMP family and is primarily located in lysosomes and endosomes, with a smaller amount on the cell surface. As a type I integral membrane protein, CD68 possesses a heavily glycosylated extracellular domain that interacts with specific lectins or selectins. It acts as a scavenger receptor, playing a role in clearing cellular debris, facilitating phagocytosis, and mediating the recruitment and activation of macrophages. The CD68 gene can undergo alternative splicing, resulting in various transcripts that encode different protein isoforms.
Description
Recombinant human CD68 protein, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 307 amino acids (residues 22-319), resulting in a molecular mass of 32.6 kDa. However, on SDS-PAGE analysis, it appears at a size of approximately 57-70 kDa due to glycosylation. The recombinant CD68 protein has a C-terminal 6-His tag for purification and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The CD68 protein is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4 with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the CD68 protein solution can be stored at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the CD68 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Macrosialin, CD68, Gp110, Macrosialin isoform A, GP110, LAMP4, SCARD1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPNDCPHKK SATLLPSFTV TPTVTESTGT TSHRTTKSHK TTTHRTTTTG TTSHGPTTAT HNPTTTSHGN VTVHPTSNST ATSQGPSTAT HSPATTSHGN ATVHPTSNST ATSPGFTSSA HPEPPPPSPS PSPTSKETIG DYTWTNGSQP CVHLQAQIQI RVMYTTQGGG EAWGISVLNP NKTKVQGSCE GAHPHLLLSF PYGHLSFGFM QDLQQKVVYL SYMAVEYNVS FPHAAQWTFS AQNASLRDLQ APLGQSFSCS NSSIILSPAV HLDLLSLRLQ AAQLPHTGVF GQSFSCPSDR SHHHHHH.

Product Science Overview

Structure and Function

CD68 is a Type I transmembrane glycoprotein with a heavily glycosylated extracellular domain . The primary sequence of human CD68 consists of 354 amino acids, with a predicted molecular weight of 37.4 kD if it were not glycosylated . The extracellular domain contains 9 potential N-linked glycosylation sites and numerous potential O-linked glycosylation sites . This domain is divided into two distinct regions separated by an extended proline hinge: a distal mucin-like domain and a proximal domain with significant sequence homology to a family of lysosomal/plasma membrane shuttling proteins .

CD68 is a member of the LAMP (lysosome-associated membrane glycoprotein) family, which includes other proteins such as LAMP1 . It is also functionally and evolutionarily related to other hematopoietic mucin-like molecules, including leukosialin/CD43 and stem cell antigen CD34 .

Expression and Localization

CD68 is primarily localized to lysosomes and endosomes, with a smaller fraction circulating to the cell surface . It is highly expressed by human monocytes and tissue macrophages . The protein plays a crucial role in the clearance of cellular debris, promotion of phagocytosis, and recruitment and activation of macrophages .

Recombinant CD68 (sf9)

Recombinant CD68 (Human) produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 307 amino acids (22-319) and has a molecular mass of 32.6 kDa . The molecular size on SDS-PAGE appears at approximately 57-70 kDa .

Applications in Research and Pathology

CD68 is widely used as a marker for various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts . Immunohistochemistry can identify the presence of CD68 in the cytoplasmic granules of a range of different blood cells and myocytes . This makes it particularly useful in distinguishing diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukemia .

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