CD68 Human

CD68 Human Recombinant
Cat. No.
BT30351
Source
E.coli.
Synonyms
Macrosialin, Gp110, CD68 antigen, CD68, SCARD1, DKFZp686M18236.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD68 Human Recombinant produced in E. coli is a single polypeptide chain containing 323 amino acids (22-319) and having a molecular mass of 34.1 kDa.
CD68 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD68 is a gene that provides instructions for creating the CD68 protein, a 110-kD transmembrane glycoprotein found in high concentrations within human monocytes and tissue macrophages. This protein is part of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and is primarily located in lysosomes and endosomes, although a small amount circulates on the cell surface. As a type I integral membrane protein, CD68 possesses a heavily glycosylated extracellular domain that binds to tissue- and organ-specific lectins or selectins. Furthermore, it belongs to the scavenger receptor family, which are known for their roles in clearing cellular debris, promoting phagocytosis, and mediating the recruitment and activation of macrophages. Alternative splicing of the CD68 gene can result in the production of multiple transcripts that encode different isoforms of the protein.
Description
Recombinant human CD68, produced in E. coli, is a single polypeptide chain comprising 323 amino acids (residues 22-319) with a molecular weight of 34.1 kDa. This protein features a 25 amino acid His-tag fused at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CD68 solution is provided at a concentration of 1mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the vial refrigerated at 4°C. For long-term storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage periods. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the CD68 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Macrosialin, Gp110, CD68 antigen, CD68, SCARD1, DKFZp686M18236.
Source
E.coli.
Amino Acid Sequence
MGSSHPLHHH SSGLVPRGSH MGSHMNDCPH KKSATLLPSF TVTPTVTEST GTTSHRTTKS HKTTTHRTTT TGTTSHGPTT ATHNPTTTSH GNVTVHPTSN STATSQGPST ATHSPATTSH GNATVHPTSN STATSPGFTS SAHPEPPPPS PSPSPTSKET IGDYTWTNGS QPCVHLQAQI QIRVMYTTQG GGEAWGISVL NPNKTKVQGS CEGAHPHLLL SFPYGHLSFG FMQDLQQKVV YLSYMAVEYN VSFPHAAQWT FSAQNASLRD LQAPLGQSFS CSNSSIILSP AVHLDLLSLR LQAAQLPHTG VFGQSFSCPS DRS

Product Science Overview

Structure and Expression

CD68 is characterized by its high degree of glycosylation, which contributes to its molecular weight of approximately 110 kDa . The protein is primarily located on the plasma membrane, as well as on endosomal and lysosomal membranes . Its expression is commonly used as a marker for monocytes and macrophages in immunohistochemical and flow cytometry analyses .

Biological Functions

CD68 plays a crucial role in the immune system. It is involved in the binding and internalization of oxidized low-density lipoprotein (OxLDL), which is important for the clearance of cellular debris and pathogens . Additionally, CD68 has been observed to form homodimers, which may be relevant to its function in phagocytosis .

Recombinant CD68

Recombinant CD68 is produced by cloning the human CD68 gene into an expression vector, which is then introduced into a suitable host cell line for protein production . The recombinant protein is typically purified using affinity chromatography techniques to ensure high purity and functionality .

Applications

Recombinant CD68 is widely used in research to study the biology of monocytes and macrophages. It is also employed in the development of diagnostic tools and therapeutic strategies for diseases involving these immune cells . For instance, CD68 antibodies are used in immunohistochemistry to identify macrophages in tissue samples, aiding in the diagnosis of various inflammatory and neoplastic conditions .

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