CD31 FITC Antibody

CD31 is characterized by its six extracellular Ig-like domains, a single transmembrane domain, and a cytoplasmic tail. This structure allows CD31 to play a crucial role in various physiological processes, such as:

• Cell adhesion: Facilitating interactions between endothelial cells and leukocytes.
• Angiogenesis: Promoting the formation of new blood vessels.
• Wound healing: Contributing to tissue repair and regeneration.
• Immune response: Modulating leukocyte transmigration during inflammatory responses .

Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used to label antibodies for flow cytometry and other fluorescence-based techniques. FITC conjugation to CD31 antibodies allows for the visualization and quantification of CD31 expression on various cell types. The FITC molecule absorbs light at a wavelength of 488 nm and emits fluorescence at around 530 nm, making it suitable for use with standard flow cytometry equipment .

CD31-FITC, Mouse Anti-Human antibodies are widely used in research and clinical settings for:

• Flow cytometry: Analyzing the expression of CD31 on different cell populations, such as peripheral blood mononuclear cells, granulocytes, and endothelial cells .
• Immunohistochemistry: Detecting CD31 expression in tissue sections to study angiogenesis, tumor vasculature, and other pathological conditions.
• Cell sorting: Isolating CD31-positive cells for further analysis or experimentation .

CD31-FITC antibodies should be stored undiluted at 2-8°C and protected from prolonged exposure to light. It is essential to avoid freezing the antibody solution, as this can lead to the formation of aggregates and loss of activity. Additionally, the presence of sodium azide in the storage buffer requires careful handling and disposal to prevent the formation of explosive compounds in plumbing systems .