CD31 Antibody

CD31, also known as Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1), is a 130-140 kDa single-chain integral membrane glycoprotein. It is a member of the immunoglobulin gene superfamily and is composed of six extracellular immunoglobulin-like domains belonging to the C2 group . CD31 is expressed by various cell types, including endothelial cells, platelets, monocytes, neutrophils, and some T cells .

CD31 plays a crucial role in modulating cell adhesion, endothelial cell migration, and angiogenesis . It is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli, such as mechanical or oxidative stress . This phosphorylation mediates associations with several SH2 domain-containing binding partners, including PI3 kinase, SHIP, PLCγ, and SHP-2 . Thus, CD31 serves as a scaffold for various signaling molecules, facilitating intracellular communication and signaling pathways .

CD31 antibodies, particularly mouse anti-human CD31 monoclonal antibodies, are widely used in scientific research. These antibodies can be used in several applications, including:

• Immunohistochemistry (IHC): Detecting CD31 expression in tissue samples.
• Flow Cytometry: Analyzing CD31 expression on cell surfaces.
• Western Blotting: Identifying CD31 protein levels in cell extracts.
• Immunocytochemistry (ICC): Visualizing CD31 in cultured cells .

Mouse anti-human CD31 monoclonal antibodies are highly specific and sensitive. They detect endogenous levels of total CD31 protein and do not cross-react with other related proteins . These antibodies are produced by immunizing animals with recombinant human CD31 protein .

CD31 antibodies are typically supplied in a buffer containing sodium HEPES, NaCl, BSA, glycerol, and sodium azide. They should be stored at -20°C and should not be aliquoted to maintain their stability and effectiveness .