CD31 Biotin Antibody

CD31 is composed of six extracellular immunoglobulin-like domains, a single transmembrane domain, and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These ITIMs are involved in signal transduction and mediate inhibitory signals that regulate cellular activation . The primary ligands for CD31 include CD38 and the vitronectin receptor (αvβ3 integrin, CD51/CD61) .

The Mouse Anti-Human CD31 antibody is a monoclonal antibody that specifically binds to human CD31. This antibody is often biotinylated to facilitate detection and purification processes. Biotinylation involves the covalent attachment of biotin to the antibody, allowing it to bind to streptavidin or avidin-conjugated detection systems, such as horseradish peroxidase (HRP) or fluorescent dyes .

Applications of CD31-Biotinylated, Mouse Anti-Human antibody include:

• Flow Cytometry: Used to analyze the expression of CD31 on various cell types.
• Immunohistochemistry: Employed to visualize CD31 expression in tissue sections, particularly in endothelial cells of blood vessels .
• Immunoprecipitation: Utilized to isolate CD31 protein complexes from cell lysates for further analysis .
• Blocking Studies: Applied in functional assays to block CD31-mediated cell-cell interactions .

The Mouse Anti-Human CD31 antibody is typically purified from tissue culture supernatant or ascites using affinity chromatography. The biotinylated form is prepared by conjugating the purified antibody with biotin under optimal conditions. The antibody solution is stored undiluted at 2°C to 8°C and should not be frozen to maintain its stability and functionality .

The storage buffer for the antibody often contains sodium azide as a preservative. Sodium azide can form highly toxic hydrazoic acid under acidic conditions, so it should be diluted in running water before disposal to prevent the accumulation of explosive deposits in plumbing .