CD2 Human, GST
Description
The CD2 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CD2, also known as Cluster of Differentiation 2, is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It plays a crucial role in the immune response by facilitating cell-cell interactions and signaling. The recombinant form of CD2, tagged with Glutathione S-Transferase (GST), is widely used in research for various applications, including protein purification and interaction studies.
CD2 is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is primarily expressed on T cells, NK cells, and thymocytes. The molecule consists of an extracellular domain, a single transmembrane region, and a cytoplasmic tail. The extracellular domain of CD2 interacts with other adhesion molecules, such as CD58 (LFA-3) in humans, to mediate cell-cell adhesion .
In addition to its adhesive properties, CD2 acts as a co-stimulatory molecule, enhancing the activation and proliferation of T cells and NK cells. This co-stimulatory function is essential for the effective immune response against pathogens .
The GST tag is a widely used protein tag for the purification and detection of recombinant proteins. GST, or Glutathione S-Transferase, is an enzyme that binds to glutathione, a tripeptide. The GST tag is typically fused to the N-terminus of the protein of interest, promoting its expression and solubility .
The primary advantages of using a GST tag include:
- Enhanced Solubility: GST promotes proper folding and solubility of the fusion protein, reducing the likelihood of aggregation and misfolding .
- Efficient Purification: The GST tag allows for easy purification of the recombinant protein using glutathione affinity chromatography. The fusion protein can be eluted from the column by adding excess reduced glutathione .
- Versatility: GST-tagged proteins can be used in various applications, including pull-down assays, protein-protein interaction studies, and enzyme activity assays .
The recombinant CD2 protein tagged with GST is utilized in several research areas:
- Immunology: Studying the role of CD2 in T cell and NK cell activation and signaling.
- Protein-Protein Interactions: Investigating interactions between CD2 and other cell surface molecules, such as CD58.
- Drug Development: Screening for potential therapeutic agents that target CD2-mediated cell adhesion and signaling pathways.
- Structural Biology: Analyzing the structure and function of CD2 through techniques like X-ray crystallography and NMR spectroscopy.
