CD2 Human, Sf9

The human recombinant CD2 protein produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 194 amino acids (25-209a.a.) and has a molecular mass of 22.3 kDa . The molecular size on SDS-PAGE appears at approximately 28-40 kDa . This recombinant protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

CD2 functions as a receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X) . Upon stimulation, CD2 leads to the tyrosine phosphorylation of several proteins, including p56lck, p59fyn, CD3eta, and CD3epsilon . This phosphorylation cascade is essential for T cell activation and the subsequent immune response.

Recombinant CD2 protein is widely used in research to study T cell differentiation and activation. It is also utilized in various assays to understand the mechanisms of immune responses and to develop potential therapeutic strategies targeting T cell-mediated diseases .

The CD2 protein solution is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol and is sterile-filtered . It has a purity greater than 95.0% as determined by SDS-PAGE . For long-term storage, it is recommended to store the protein at -20°C with a carrier protein to avoid multiple freeze-thaw cycles .

ProSpec’s CD2 Human Recombinant protein is intended for laboratory research use only and is not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals . Proper safety measures should be taken while handling this protein to ensure laboratory safety.