CD2 FITC Antibody
Description
Product Specs
Product Science Overview
CD2-FITC (T11, LFA-2), Mouse Anti-Human FITC is a monoclonal antibody used in immunological research and diagnostics. This antibody is conjugated with fluorescein isothiocyanate (FITC), a fluorescent dye, which allows for the visualization of CD2 expression on the surface of human cells through techniques such as flow cytometry.
CD2, also known as T11, LFA-2 (Lymphocyte Function-associated Antigen-2), or the sheep red blood cell receptor (SRBC-R), is a 50 kDa type I transmembrane glycoprotein. It is a member of the immunoglobulin superfamily and is primarily expressed on thymocytes, T lymphocytes, natural killer (NK) cells, and subsets of thymic B cells .
The primary function of CD2 is to mediate cell-cell adhesion and facilitate T cell activation and signaling. CD2 interacts with its major ligand, CD58 (LFA-3), as well as other ligands such as CD48, CD59, and CD15 . These interactions are crucial for the formation of the immunological synapse and the subsequent activation of T cells.
Fluorescein isothiocyanate (FITC) is a widely used fluorescent dye that is conjugated to antibodies for the purpose of detecting specific antigens. FITC emits a bright green fluorescence when excited by light of a specific wavelength, making it an ideal marker for flow cytometry and other fluorescence-based assays .
CD2-FITC (T11, LFA-2), Mouse Anti-Human FITC is commonly used in flow cytometry to identify and quantify CD2-expressing cells in human samples. This can be particularly useful in immunophenotyping, where the presence and proportion of different immune cell subsets are analyzed. Additionally, it can be used in research to study T cell activation, signaling pathways, and cell-cell interactions .
The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. It should not be frozen, as this can affect the stability and functionality of the antibody. Proper handling and disposal procedures should be followed, as the reagent contains sodium azide, which can be hazardous .
