CD226 Human, Sf9

CD226 Human Recombinant, Sf9
Cat. No.
BT26875
Source
Sf9, Baculovirus cells.
Synonyms
CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226 Molecule, CD226 Antigen, DNAX Accessory Molecule 1, DNAM-1, DNAM1, T Lineage-Specific Activation Antigen 1 Antigen, Platelet And T Cell Activation Antigen 1, DNAX Accessory Molecule-1, Adhesion Glycoprotein, TLiSA1, PTA1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

CD226 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 471 amino acids (19-247a.a) and having a molecular mass of 53.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
CD226 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD226, a member of the immunoglobulin superfamily, is a 65kDa glycoprotein found on the surface of NK cells, platelets, monocytes, and a subset of T cells. This protein plays a crucial role in cell adhesion, specifically facilitating the binding of platelets and megakaryocytic cells to vascular endothelial cells. Additionally, CD226 contributes to the maturation process of megakaryocytes.
Description
Recombinant human CD226, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 471 amino acids (spanning from amino acid 19 to 247a) and has a molecular weight of 53.2 kDa. Note that on SDS-PAGE, the apparent molecular size may appear between 50-70 kDa. For purification purposes, the CD226 protein is tagged with a 239 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The CD226 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is formulated with Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the product refrigerated at 4°C. For longer-term storage, freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the CD226 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226 Molecule, CD226 Antigen, DNAX Accessory Molecule 1, DNAM-1, DNAM1, T Lineage-Specific Activation Antigen 1 Antigen, Platelet And T Cell Activation Antigen 1, DNAX Accessory Molecule-1, Adhesion Glycoprotein, TLiSA1, PTA1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPEEVLWHT SVPFAENMSL ECVYPSMGIL TQVEWFKIGT QQDSIAIFSP THGMVIRKPY AERVYFLNST MASNNMTLFF RNASEDDVGY YSCSLYTYPQ GTWQKVIQVV QSDSFEAAVP SNSHIVSEPG KNVTLTCQPQ MTWPVQAVRW EKIQPRQIDL LTYCNLVHGR NFTSKFPRQI VSNCSHGRWS VIVIPDVTVS DSGLYRCYLQ ASAGENETFV MRLTVAEGKT DNLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

Product Science Overview

Introduction

CD226, also known as DNAX Accessory Molecule-1 (DNAM-1), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed on the surface of various immune cells, including T cells, natural killer (NK) cells, monocytes, and platelets . CD226 plays a crucial role in the immune response, particularly in the context of tumor immunology and autoimmune diseases .

Structure and Function

CD226 is composed of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain contains two immunoglobulin-like domains, which are responsible for binding to its ligands, CD155 (PVR) and CD112 (Nectin-2) . The interaction between CD226 and its ligands promotes the activation and cytotoxicity of NK cells and T cells, enhancing their ability to target and destroy tumor cells .

Role in Cancer Immunotherapy

CD226 has emerged as a potent driver of antitumor immunity. It competes with inhibitory receptors such as TIGIT and CD96 for binding to CD155 and CD112 . The balance between activating and inhibitory signals mediated by these receptors is critical for the regulation of immune responses against tumors. High expression levels of CD226 on tumor-infiltrating lymphocytes are associated with improved antitumor responses and better patient outcomes .

Recent studies have shown that the expression of CD226 can be downregulated in tumors, leading to reduced immune surveillance and tumor progression . Strategies to maintain or enhance CD226 expression on immune cells are being explored as potential therapeutic approaches to boost antitumor immunity .

Recombinant CD226 (Human, Sf9)

Recombinant CD226 (Human, Sf9) refers to the human CD226 protein produced using the Sf9 insect cell expression system. This system is commonly used for the production of recombinant proteins due to its high yield and post-translational modification capabilities. The recombinant CD226 protein can be used in various research applications, including studies on immune cell activation, cancer immunotherapy, and autoimmune diseases.

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