CD8 Antibody
CD8, Rat Anti-Mouse
Cat. No.
BT4283
Source
Synonyms
CD8, MAL, p32.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
CD8 is a glycoprotein expressed on the surface of most cytotoxic T lymphocytes, playing a crucial role in cell-cell interactions within the immune system. It functions as a co-receptor alongside the T-cell receptor on the T lymphocyte, facilitating the recognition of antigens presented by antigen-presenting cells (APCs) in the context of class I MHC molecules. CD8 exists as either a homodimer of two alpha chains or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains exhibit significant structural similarity to immunoglobulin variable light chains. The primary function of CD8 is to identify cytotoxic/suppressor T cells that engage with MHC class I-bearing target cells. It is believed to be involved in the process of T-cell-mediated cell killing. Notably, CD8 alpha chains bind to the alpha-3 domains of class I MHC molecules.
Formulation
Supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) following reconstitution.
Storage Procedures
The lyophilized product should be stored at 4°C. After reconstitution, it is recommended to aliquot and store the antibody at -20°C if not intended for use within one month.
Solubility
To reconstitute, add the appropriate volume of deionized water (H2O) to the vial. Gently mix the solution, ensuring the sides of the vial are washed, and allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for use in cytotoxicity assays and cell staining applications. For staining procedures, a concentration of 10 µl per 1,000,000 cells is recommended. The optimal titer for cytotoxicity assays should be empirically determined by the investigator.
Available Conjugates
This antibody is currently available conjugated to Biotin and Fluorescein isothiocyanate (FITC).
Synonyms
CD8, MAL, p32.
Purification Method
Ion Exchange Column.
Type
Rat Anti Mouse Monoclonal.
Clone
NYRmCD8.
Immunogen
Purified Mouse LN T cells.
Ig Subclass
Rat IgM.
Product Science Overview
CD8 Structure and Function
- Structure: The CD8 molecule consists of two chains, CD8α and CD8β. The CD8α chain can pair with either another CD8α chain or a CD8β chain to form a homodimer or heterodimer, respectively . The CD8α and CD8β chains are encoded by separate genes and are expressed on the surface of T cells.
- Function: CD8 acts as a co-receptor for the T cell receptor (TCR) and enhances the sensitivity of T cells to antigens presented by MHC class I molecules. This interaction is essential for the recognition and elimination of infected or malignant cells by cytotoxic T lymphocytes (CTLs) .
Rat Anti-Mouse CD8 Antibody
The Rat Anti-Mouse CD8 antibody is a monoclonal antibody that specifically binds to the CD8α chain on mouse cells. This antibody is commonly used in immunological research to study T cell populations and their functions.
- Clone: One of the well-known clones of Rat Anti-Mouse CD8 is the 53-6.7 clone . This clone specifically binds to the 38 kDa α and 34 kDa α’ chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested .
- Applications: The Rat Anti-Mouse CD8 antibody is widely used in flow cytometry, immunohistochemistry, and other immunological assays to identify and quantify CD8+ T cells in various tissues .
Importance in Research
The use of Rat Anti-Mouse CD8 antibodies has significantly advanced our understanding of the immune system, particularly in the context of T cell biology. These antibodies have been instrumental in:
- Characterizing T Cell Subsets: By using Rat Anti-Mouse CD8 antibodies, researchers can distinguish between different subsets of T cells, such as cytotoxic T lymphocytes (CTLs) and helper T cells .
- Studying Immune Responses: These antibodies help in studying the immune responses in various disease models, including infections, cancer, and autoimmune diseases .
- Therapeutic Development: Insights gained from research using Rat Anti-Mouse CD8 antibodies contribute to the development of immunotherapies and vaccines .
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