CD8 Anti-Human
CD8, Mouse Anti-Human
Cat. No.
BT2170
Source
Synonyms
CD8, MAL, p32.
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Introduction
CD8 is a glycoprotein present on the surface of most cytotoxic T lymphocytes. It plays a crucial role in cell-cell interactions within the immune system by acting as a co-receptor for the T-cell receptor (TCR). CD8, in conjunction with the TCR, recognizes antigens presented by antigen-presenting cells (APCs) in the context of class I MHC molecules. Functionally, CD8 exists either as a homodimer of two alpha chains or a heterodimer of one alpha and one beta chain. Both the alpha and beta chains share significant structural similarities with immunoglobulin variable light chains. CD8 is a marker for cytotoxic/suppressor T-cells that interact with MHC class I-bearing target cells and is believed to be involved in T-cell-mediated killing. The alpha chains of CD8 bind to the alpha-3 domains of class I MHC molecules.
Formulation
The antibody is supplied as a solution with a concentration of 1 milligram per milliliter in phosphate-buffered saline (PBS) after reconstitution.
Storage Procedures
The lyophilized product should be stored at a temperature of 4 degrees Celsius. Upon reconstitution, it is recommended to use the antibody within one month. If not used within this timeframe, it is advisable to aliquot the reconstituted antibody into smaller volumes and store them at -20 degrees Celsius.
Solubility
To reconstitute the lyophilized antibody, add the recommended volume of sterile water (H2O). Mix the solution gently by inverting the vial several times. Rinse the sides of the vial with the reconstituted antibody solution. Allow the solution to sit undisturbed for 30-60 seconds before use to ensure complete reconstitution.
Applications
This antibody can be used for blocking experiments and staining procedures. For staining applications, it is recommended to use 10 microliters of the antibody per 1,000,000 cells. The optimal titer for blocking T cell activation should be determined empirically by the investigator.
Available Conjugates
In addition to the unconjugated form, this antibody is also available conjugated to biotin and fluorescein isothiocyanate (FITC). For staining experiments using the biotin- or FITC-conjugated antibody, it is recommended to use 5-10 microliters of the antibody per 1,000,000 cells.
Synonyms
CD8, MAL, p32.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Clone
hCD8.
Immunogen
Purified human PBL CD8+ T cells.
Ig Subclass
Mouse IgG2a.
Product Science Overview
Structure and Function
CD8 molecules are part of the immunoglobulin superfamily and are involved in the recognition of antigens presented by MHC class I molecules. The CD8α chain binds to the α3 domain of MHC class I, while the cytoplasmic tail of CD8 interacts with the tyrosine kinase p56 Lck, which is essential for T cell activation .
Applications
Anti-CD8 antibodies, particularly those developed in mice, are widely used in various scientific applications, including:
Mouse Anti-Human CD8 Antibodies
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