CD8 Biotin Antibody
Description
Product Specs
Product Science Overview
The “CD8, Rat Anti-Mouse, Biotin” antibody is a monoclonal antibody specifically designed to target the CD8 antigen in mice. This antibody is conjugated with biotin, a vitamin that allows for easy detection and purification of the antibody-antigen complex. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, which play a crucial role in the immune response.
The CD8 antigen, also known as Lyt-2 or Ly-2, consists of disulfide-linked alpha (α) and beta (β) chains that form either an α/β heterodimer or an α/α homodimer. The CD8 α/β heterodimer is predominantly expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. The CD8 α/α homodimer, on the other hand, is expressed on a subset of γ/δ TCR-bearing T cells, natural killer (NK) cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells .
CD8 serves as an antigen co-receptor on T cells, interacting with major histocompatibility complex (MHC) class I molecules on antigen-presenting cells or epithelial cells. This interaction is crucial for the activation of T cells, as it promotes the association of the T-cell receptor (TCR) complex with protein tyrosine kinase lck (p56lck), leading to T-cell activation and subsequent immune response .
The rat anti-mouse CD8 antibody is a monoclonal antibody that specifically binds to the CD8 antigen in mice. The antibody is produced by immunizing rats with mouse thymus or spleen cells, followed by the extraction and purification of the antibody from the rat’s serum. The antibody is then conjugated with biotin, which allows for easy detection and purification using streptavidin-based methods .
The biotin-conjugated rat anti-mouse CD8 antibody is widely used in various immunological assays, including:
- Flow Cytometry: This technique allows for the analysis of cell surface markers, such as CD8, on individual cells within a mixed population. The biotin-conjugated antibody can be detected using streptavidin-conjugated fluorochromes, enabling the identification and quantification of CD8-expressing cells .
- Cell Separation: The biotin-conjugated antibody can be used to isolate CD8-expressing cells from a mixed population using streptavidin-coated magnetic beads or columns .
- Immunohistochemistry: This technique involves the staining of tissue sections to visualize the distribution and localization of CD8-expressing cells. The biotin-conjugated antibody can be detected using streptavidin-conjugated enzymes or fluorochromes .
