CCL3L1 Human, His

LD78-beta (CCL3L1) Human Recombinant, His Tag
Cat. No.
BT16493
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 3-like 1, G0/G1 switch regulatory protein 19-2, LD78-beta(1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein, CCL3L1, D17S1718, G0S19-2, SCYA3L1, LD78, 464.2, SCYA3L, LD78BETA, MGC12815.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCL3L1 Human Recombinant fused with a 22 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 90 amino acids (26-93 a.a.) and having a molecular mass of 10kDa. The CCL3L1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
CCL3L1, a small cytokine belonging to the CC chemokine family, is encoded by a gene located on chromosome 17q, among a cluster of cytokine genes. This chemokine plays a crucial role in immune regulation and inflammatory responses. CCL3L1 interacts with several chemokine receptors, notably CCBP2 and CCR5. The latter is known to act as a co-receptor for HIV, and CCL3L1 binding to CCR5 can inhibit HIV entry.
Description
Recombinant human CCL3L1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein, with a molecular weight of 10 kDa, comprises 90 amino acids (26-93 a.a.), including a 22 amino acid His tag at the N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CCL3L1 solution is provided at a concentration of 0.25 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
C-C motif chemokine 3-like 1, G0/G1 switch regulatory protein 19-2, LD78-beta(1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein, CCL3L1, D17S1718, G0S19-2, SCYA3L1, LD78, 464.2, SCYA3L, LD78BETA, MGC12815.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA.

Product Science Overview

Introduction

LD78-beta, also known as CCL3L1, is a member of the CC chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells. The primary function of chemokines is to induce chemotaxis in nearby responsive cells, particularly immune cells. LD78-beta is closely related to macrophage inflammatory protein 1-alpha (MIP-1α) and plays a significant role in inflammatory and immunoregulatory processes .

Gene and Protein Structure

The gene encoding LD78-beta is located on the q-arm of chromosome 17. This gene is part of a cluster of cytokine genes. The protein encoded by this gene binds to several chemokine receptors, including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). The CCR5 receptor is notably a co-receptor for HIV, and binding of LD78-beta to CCR5 inhibits HIV entry .

Recombinant human LD78-beta is a 7.7 kDa protein containing 70 amino acid residues, including the four conserved cysteine residues present in CC chemokines. The protein is typically expressed in Escherichia coli (E. coli) and purified to a high degree of purity, often greater than 98% as determined by SDS-PAGE and HPLC analyses .

Biological Activity

LD78-beta exhibits potent activity in HIV suppression assays. It signals through the CCR5 receptor and the beta-chemokine receptor, D6. The ability of LD78-beta to bind to CCR5 and inhibit HIV entry makes it a molecule of significant interest in HIV research .

Preparation Methods

Recombinant LD78-beta is typically produced using bacterial expression systems, such as E. coli. The gene encoding LD78-beta is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the recombinant protein is expressed. Following expression, the protein is purified using techniques such as affinity chromatography, which exploits the His tag attached to the protein for purification .

Applications

LD78-beta is used in various research applications, including:

  • HIV Research: Due to its ability to inhibit HIV entry by binding to CCR5, LD78-beta is extensively studied in the context of HIV infection and potential therapeutic interventions.
  • Immunology: As a chemokine, LD78-beta is involved in the regulation of immune cell trafficking and activation, making it a valuable tool in immunological studies.
  • Inflammation: LD78-beta’s role in inflammatory processes makes it relevant in research focused on understanding and treating inflammatory diseases .

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CCL3L1 Human
LD78-beta (CCL3L1) Human Recombinant
Cat. No.
BT16430
Source
Escherichia Coli.
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