CCL3L1 Human
LD78-beta (CCL3L1) Human Recombinant
Cat. No.
BT16430
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 3-like 1, G0/G1 switch regulatory protein 19-2, LD78-beta(1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein, CCL3L1, D17S1718, G0S19-2, SCYA3L1, LD78, 464.2, SCYA3L, LD78BETA, MGC12815.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CCL3L1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. The CCL3L1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CCL3L1, a small cytokine belonging to the CC chemokine family, is encoded by a gene located on chromosome 17q, clustered among other cytokine genes. This chemokine plays a crucial role in immune regulation and inflammatory responses. CCL3L1 interacts with multiple chemokine receptors, including CCBP2 and CCR5. Notably, CCR5 serves as a co-receptor for HIV, and CCL3L1 binding to CCR5 can inhibit HIV entry.
Description
Recombinant human CCL3L1, produced in E. coli, is a single-chain polypeptide. This non-glycosylated protein comprises 70 amino acids, resulting in a molecular weight of 7.7 kDa. The purification of CCL3L1 is achieved through proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
Lyophilized from a 0.2 µm filtered solution concentrated in 1xPBS, pH 7.4.
Solubility
Reconstitute the lyophilized CCL3L1 in sterile 18 MΩ·cm H₂O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
While lyophilized CCL3L1 remains stable at room temperature for up to 3 weeks, storage in desiccated conditions below -18°C is recommended. After reconstitution, store CCL3L1 at 4°C for 2-7 days. For long-term storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 97.0% as determined by: (a) RP-HPLC analysis, and (b) SDS-PAGE analysis.
Biological Activity
Biological activity is evaluated based on the chemokine's capacity to chemoattract human monocytes within a concentration range of 1.0-10.0 ng/ml.
Synonyms
C-C motif chemokine 3-like 1, G0/G1 switch regulatory protein 19-2, LD78-beta(1-70), PAT 464.2, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein, CCL3L1, D17S1718, G0S19-2, SCYA3L1, LD78, 464.2, SCYA3L, LD78BETA, MGC12815.
Source
Escherichia Coli.
Amino Acid Sequence
APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA.
Product Science Overview
Genetic and Structural Information
- Gene Location: The gene encoding LD78-beta, CCL3L1, is located on chromosome 17q12. It is part of a segmental duplication region that is rich in immune response genes .
- Protein Structure: LD78-beta is a 7.8 kDa protein consisting of 70 amino acid residues, including four conserved cysteine residues characteristic of CC chemokines . The protein’s structure allows it to bind with high affinity to CC chemokine receptors CCR1 and CCR5 .
Biological Functions
- Chemotaxis: LD78-beta is known for its ability to attract immune cells to sites of inflammation. It has been shown to chemoattract human monocytes and BaF3 mouse pro-B cells transfected with human CCR5 .
- HIV Suppression: LD78-beta exhibits potent activity in HIV suppression assays by signaling through the CCR5 receptor, which is a co-receptor for HIV entry into cells .
Recombinant Production
- Expression Systems: Recombinant LD78-beta can be produced in various expression systems, including E. coli and HEK293 cells . The choice of expression system can affect the protein’s purity, activity, and endotoxin levels.
- Purity and Activity: Recombinant LD78-beta is typically purified to greater than 95% purity and has an endotoxin level of less than 0.01 EU per 1 μg of protein . Its biological activity is measured by its ability to chemoattract specific cell types .
Applications
- Research: LD78-beta is widely used in research to study immune responses, inflammation, and HIV infection mechanisms. Its role as a chemoattractant makes it valuable for investigating cell migration and signaling pathways.
- Therapeutic Potential: Due to its involvement in immune regulation and HIV suppression, LD78-beta holds potential for therapeutic applications in treating inflammatory diseases and HIV infection.
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