CALB1 Rat

Calbindin-1 Rat Recombinant
Cat. No.
BT28453
Source
Escherichia Coli.
Synonyms
Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Recombinant Rat Calbindin-1 produced in E.Coli.
The Rat CALB1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Calbindins are calcium-binding proteins belonging to the troponin C superfamily. CALB28K/Calbindin1/CALB1 (D28K/Spot35 protein or cholecalcin, with varying lengths of 261 amino acids in rat, mouse, and human) is located on chromosome 8q21.3-q22.1 in humans. Originally discovered as a 27-kDa protein induced by vitamin D in chicken duodenum, in mammals, it's found in the kidneys, pancreatic islets, and brain. Interestingly, its brain synthesis is vitamin D-independent. CABP28K possesses four active and two inactive EF-hand calcium-binding domains. Its gene shares a clustered region with carbonic anhydrase. Notably, neurons in individuals with Huntington's disease show a depletion of CAB28K. Two primary types of CaBPs exist: "trigger" and "buffer" CaBPs. "Trigger" CaBPs undergo conformational changes upon binding calcium, exposing protein regions that interact with and modify the activity of target molecules. In contrast, "buffer" CABPs are believed to regulate intracellular calcium levels. Calbindin D-28K is primarily localized in specific central and peripheral nervous system neurons, and in certain epithelial cells associated with calcium transport, including distal tubular cells and cortical collecting tubules of the kidney, as well as in enteric neuroendocrine cells.
Description
Recombinant Rat Calbindin-1, produced in E. coli, has been purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 1 mg/ml solution containing 50 mM NaHCO₃.
Solubility
To reconstitute the lyophilized CABP28K, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized CABP28K remains stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, CABP28K should be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is advised. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Applications
CABP28K is a versatile protein suitable for various applications, including immunoblotting, absorption experiments in immunohistochemistry, radioimmunoassay, and intracellular injection. For adsorption purposes, the following procedure is recommended: A. Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the standard buffer used for immunohistochemistry (final dilution 1:5,000). B. Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix thoroughly. C. Incubate the mixture for a minimum of 6 hours at a low temperature (e.g., 4°C). D. Apply the mixture to tissue sections and incubate for 3 days. E. Complete the immunohistochemical reaction as usual (biotinylated secondary antibody, ABC-complex, DAB). This procedure should significantly reduce or even completely eliminate immunostaining.
Synonyms
Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.
Source
Escherichia Coli.

Product Science Overview

Structure and Function

Calbindin-1 is a low molecular weight protein with a molecular weight of approximately 28 kDa. It contains six EF-hand motifs, each capable of binding a calcium ion. This protein acts as an intracellular calcium buffer, regulating the concentration of free calcium ions within the cytoplasm. By binding to calcium ions, Calbindin-1 helps to modulate various cellular processes, including neurotransmitter release, enzyme activity, and gene expression.

Expression and Localization

In the CNS, Calbindin-1 is expressed in discrete neuronal populations. It is highly abundant in Purkinje cells of the cerebellum, where it plays a critical role in motor coordination and synaptic plasticity . Additionally, Calbindin-1 is found in other brain regions, including the hippocampus, thalamus, and certain cortical areas. Its expression is developmentally regulated, with higher levels observed during periods of neuronal differentiation and migration .

Biological Significance

Calbindin-1 is essential for maintaining calcium homeostasis in neurons. It acts as a calcium buffer, preventing excessive calcium fluctuations that could lead to cellular damage. This protein also participates in calcium signaling pathways, influencing processes such as synaptic transmission and plasticity. In Purkinje cells, Calbindin-1 is involved in long-term depression (LTD), a form of synaptic plasticity critical for motor learning .

Recombinant Calbindin-1

Recombinant Calbindin-1 (Rat) is produced using recombinant DNA technology, allowing for the expression and purification of the protein in a controlled laboratory setting. This recombinant form is used in various research applications, including studies on calcium signaling, neurobiology, and protein-protein interactions. The availability of recombinant Calbindin-1 enables researchers to investigate its biochemical properties and functional roles in greater detail.

Research Applications

Calbindin-1 is widely used as a marker for specific neuronal populations in immunohistochemistry and immunofluorescence studies. Its expression pattern helps to identify and characterize different types of neurons within the CNS. Additionally, Calbindin-1 is utilized in studies exploring the mechanisms of calcium signaling and its impact on neuronal function and plasticity .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

S100G Rat
S100 Calcium Binding Protein G Rat Recombinant
Cat. No.
BT1752
Source
Escherichia Coli.
SCGN Rat
Secretagogin Rat Recombinant
Cat. No.
BT19947
Source
Escherichia Coli.
CALB1 Human
Calbindin-1 Human Recombinant
Cat. No.
BT28364
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.