CALB1 Human
Description
The CALB1 is purified by proprietary chromatographic techniques.
Product Specs
CALB1 possesses four active calcium-binding domains and two modified domains that seem to have lost their calcium-binding capacity. Its expression is primarily observed in neural tissues, with brain synthesis being independent of vitamin-D-derived hormones.
Dysregulation of CALB1 has been implicated in neurological disorders such as epilepsy, amyotrophic lateral sclerosis, and Huntington's disease. Notably, neurons in the brains of Huntington's disease patients exhibit CALB1 depletion.
To ensure long-term stability, adding a carrier protein such as HSA or BSA (0.1%) is advised.
Repeated freezing and thawing should be avoided.
Product Science Overview
Calbindin-1 is a 28 kDa protein that contains four active calcium-binding domains and two modified domains that have lost their calcium-binding capability . This protein acts as a calcium buffer and calcium sensor, capable of holding four Ca²⁺ ions in the EF-hands of loops EF1, EF3, EF4, and EF5 . It is thought to buffer the entry of calcium upon stimulation of glutamate receptors .
Calbindin-1 is expressed in discrete neuronal populations within the central nervous system (CNS) and is believed to act as an intracellular calcium-buffering protein . Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate . This protein plays a crucial role in regulating calcium levels within neurons, which is essential for various cellular processes.
Recombinant calbindin-1 is produced using recombinant DNA technology, which involves inserting the CALB1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications to study its structure, function, and role in different biological processes.
