CAIII Human, His
Greater than 95.0% as determined by SDS-PAGE.
Description
The CA-III is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Carbonic anhydrase III (CAIII) is a member of the carbonic anhydrase family, a group of metalloenzymes that catalyze the reversible hydration of carbon dioxide. This reaction is crucial for various physiological processes, including respiration and acid-base balance. CAIII is a cytoplasmic isoenzyme predominantly found in skeletal muscle and, to a lesser extent, in cardiac and smooth muscle .
CAIII is characterized by its ability to catalyze the conversion of carbon dioxide and water to bicarbonate and protons. This reaction is essential for maintaining pH balance in tissues and facilitating the transport of carbon dioxide out of tissues. The enzyme’s active site typically contains a zinc ion, which is crucial for its catalytic activity .
Human recombinant CAIII is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is expressed with an amino-terminal hexahistidine (His) tag, which facilitates its purification through affinity chromatography techniques. The molecular mass of the recombinant CAIII is approximately 33.9 kDa .
Recombinant CAIII is widely used in research to study its role in various physiological and pathological processes. It serves as a positive control in immunological assays and is used to investigate the enzyme’s function in muscle physiology and its involvement in diseases such as Duchenne muscular dystrophy .
The recombinant CAIII is supplied in a buffer containing Tris-HCl, NaCl, DTT, cysteine, and glycerol. It is recommended to store the protein at 4°C if it will be used within 2-4 weeks. For long-term storage, the protein should be kept frozen at -20°C, and the addition of a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) is advised to prevent degradation .
