Introduction
Carbonic anhydrase III, a member of a multigene family encoding carbonic anhydrase isozymes, plays a crucial role in catalyzing the reversible hydration of carbon dioxide. These metalloenzymes exhibit differential expression across various cell types. Notably, CA3 demonstrates strict tissue specificity, with high expression levels observed in skeletal muscle and significantly lower levels in cardiac and smooth muscle. Functionally, CA3 facilitates the rapid conversion of carbon dioxide to bicarbonate and protons, as depicted in the following equation: CO2 + H2O = HCO3 + H+. This enzymatic activity is central to a range of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of vital fluids such as aqueous humor, cerebrospinal fluid, saliva, and gastric juice. Structurally, CA3 incorporates a zinc ion within its active site, enabling its participation in maintaining acid-base equilibrium within blood and other tissues, as well as facilitating carbon dioxide transport within tissues.
Description
Recombinant human CA3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 260 amino acids (1-260 a.a.). With a molecular mass of 29.5 kDa, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear and colorless solution.
Formulation
The CA3 solution is formulated in 20mM Tris-HCl pH 8, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Car3, CAIII, Carbonic anhydrase 3, EC 4.2.1.1, Carbonic anhydrase III, Carbonate dehydratase III, CA-III.
Amino Acid Sequence
MAKEWGYASH NGPDHWHELF PNAKGENQSP IELHTKDIRH DPSLQPWSVS YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI NNRVVRASFK.
