C3 Rabbit
Rabbit Plasma.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Sterile filtered solution.
Greater than 85% as determined by SDS-PAGE.
Description
Rabbit Complement C3 produced in Rabbit plasma having a molecular mass of 185 kDa.
Product Specs
Complement component 3 (C3) is essential for the activation of all three complement pathways: classical, lectin, and alternative. The activation process involves the formation of protease complexes that bind to target surfaces. These complexes cleave C3, generating the anaphylatoxin C3a and the activated fragment C3b. A significant portion of activated C3 doesn't bind to the target surface due to a rapid reaction of its thioester group with water, resulting in fluid-phase C3b. This fluid-phase C3b is swiftly deactivated by factors H and I, leading to the formation of iC3b. Surface-bound C3b is crucial in all three pathways for the effective activation of C5 and the subsequent assembly of C5b-9 complexes, which ultimately lyse the target cell membrane.
This product consists of Rabbit Complement C3, produced from Rabbit plasma, with a molecular weight of 185 kDa.
This product is provided as a sterile, filtered solution.
The C3 solution is formulated in a buffer containing 145 mM sodium chloride (NaCl) and 10 mM sodium phosphate, with a pH of 7.2.
Rabbit C3 remains stable for 2-4 weeks when stored at 4°C, provided the entire vial is used within this period. For extended storage, freeze the product below -20°C. It is advisable to add a carrier protein (0.1% HSA or BSA) for long-term storage. To maintain product integrity, avoid repeated freezing and thawing.
The purity of this product is greater than 85%, as determined by SDS-PAGE analysis.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Rabbit Plasma.
Product Science Overview
Complement C3 is central to both the classical and alternative pathways of complement activation .
- Classical Pathway: Triggered by antigen-antibody complexes.
- Alternative Pathway: Activated on microbial surfaces in the absence of antibodies.
Both pathways converge at the activation of C3, leading to the generation of C3a and C3b, which further propagate the immune response .
In research, Complement C3 Rabbit antibodies are used to study the expression and function of C3 in various biological contexts. These antibodies are valuable tools for techniques such as Western blotting, immunoprecipitation, and immunohistochemistry . They help in understanding the role of C3 in immune responses and its involvement in various diseases.
