C3 Mouse
Mouse Plasma.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Sterile filtered solution.
Greater than 93.0% as determined by SDS-PAGE.
Description
Mouse Complement C3 produced in Mouse plasma having a molecular weight of 185kDa.
Product Specs
Complement component 3 (C3) is pivotal in activating all three complement pathways. Each pathway's initiation generates proteolytic enzyme complexes, binding to the target surface. These enzymes cleave C3, releasing the anaphylatoxin C3a and activating C3b. Notably, most activated C3 doesn't attach to the surface; its thioester reacts with water, forming fluid-phase C3b, quickly inactivated by factors H and I into iC3b. Surface-bound C3b is crucial across all pathways for effective C5 activation and the formation of C5b-9 complexes, ultimately lysing the target cell membrane.
Murine Complement Component 3 (C3) derived from mouse plasma, exhibiting a molecular weight of 185 kDa.
A solution subjected to sterile filtration.
The C3 solution is prepared in a phosphate-buffered saline (PBS).
Mouse C3 remains stable at 4°C for 2-4 weeks, provided the entire vial is used within this period. For extended storage, freeze at temperatures below -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Minimize freeze-thaw cycles.
Purity exceeds 93.0%, as determined by SDS-PAGE analysis.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Mouse Plasma.
Product Science Overview
Complement C3 is a large glycoprotein that is synthesized primarily in the liver and then secreted into the bloodstream. It is central to the activation of all three pathways of complement activation: the classical pathway, the lectin pathway, and the alternative pathway .
- Classical Pathway: Initiated by antibodies bound to antigens.
- Lectin Pathway: Triggered by mannose-binding lectin binding to pathogen surfaces.
- Alternative Pathway: Activated directly by pathogen surfaces.
Each pathway generates proteolytic enzyme complexes known as C3 convertases, which cleave C3 into two fragments: C3a and C3b .
- C3a: Functions as an anaphylatoxin, mediating inflammatory responses.
- C3b: Plays a key role in opsonization, tagging pathogens for destruction by phagocytes. It also participates in the formation of the C5 convertase, which is essential for the assembly of the membrane attack complex (MAC) that lyses target cells .
The activation of C3 is a pivotal step in the complement cascade. The cleavage of C3 and the subsequent binding of C3b to pathogen surfaces mark the pathogens for destruction. This process is known as opsonization and is crucial for the efficient clearance of pathogens by phagocytic cells such as macrophages and neutrophils .
Additionally, the breakdown products of C3b, such as iC3b and C3d, are recognized by various receptors on immune cells. These interactions enhance antigen presentation and stimulate the adaptive immune response, leading to the expansion of antigen-specific B-cell and T-cell populations .
Complement C3 is extensively studied in immunology and pathology. Researchers use various assays, such as ELISA (Enzyme-Linked Immunosorbent Assay), to measure the levels of C3 in mouse serum and plasma samples . These studies help in understanding the role of C3 in immune responses and its involvement in various diseases, including autoimmune disorders, infections, and inflammatory conditions.
