C3 Human
Human Plasma.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Sterile Filtered solution.
Greater than 94.0% as determined by SDS-PAGE.
Description
Human Complement C3 produced in Human plasma having a molecular mass of 185 kDa.
Product Specs
Human C3, a naturally glycosylated polypeptide with two disulfide-linked chains, plays a pivotal role in activating all three complement pathways. Each pathway's initiation generates proteolytic enzyme complexes that bind to target surfaces. These enzymes cleave C3, releasing the anaphylatoxin C3a and activating C3b. Due to its thioester reaction with water, a significant portion of activated C3 doesn't bind to the surface, forming fluid phase C3b, rapidly inactivated by factors H and I into iC3b. Surface-bound C3b is crucial across all pathways for efficient C5 activation and the formation of C5b-9 complexes, which lyse target cell membranes.
Human Complement C3, derived from human plasma, has a molecular weight of 185 kDa.
The product is a sterile filtered solution.
The C3 solution is prepared in phosphate buffer saline.
Human C3 remains stable for 2-4 weeks when stored at 4°C. For extended storage, freeze below -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
The purity is determined to be greater than 94.0% using SDS-PAGE analysis.
The plasma used in this product has undergone rigorous testing and is confirmed negative for antibodies against HIV-1, HIV-2, HCV, HTLV-I/II, STS, and HBSAg.
Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, CPAMD1.
Human Plasma.
Product Science Overview
C3 is the most abundant and essential protein in the complement system. It plays a pivotal role in both the classical and alternative pathways of complement activation. When the immune system detects harmful substances, C3 is activated and undergoes a conformational change, leading to its cleavage into two fragments: C3a and C3b .
- C3a: Acts as an anaphylatoxin, which means it can induce inflammation by increasing vascular permeability and attracting immune cells to the site of infection.
- C3b: Functions as an opsonin, marking pathogens for destruction by phagocytes. It also forms part of the C5 convertase complex, which is crucial for the formation of the membrane attack complex (MAC) that can lyse pathogens .
Research into C3 and its pathways has significant therapeutic potential. Understanding the mechanisms of C3 activation and regulation can lead to the development of treatments for autoimmune diseases, infections, and other immune-related conditions. Recombinant forms of C3 are also being explored for their potential in therapeutic applications.
