Introduction
Chromosome 19 Open Reading Frame 80 (C19ORF80), a member of the ANGPTL protein family, plays a crucial role in regulating lipid metabolism, particularly serum triglyceride levels. This regulation is potentially achieved by promoting ANGPTL3 cleavage. Moreover, C19ORF80 acts as a hormone that specifically stimulates the proliferation and expansion of pancreatic beta cells, leading to improved glucose tolerance. Primarily expressed in the liver, C19ORF80 is also found in adipose tissues and its expression is influenced by food intake.
Description
Recombinant C19ORF80, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 22-198, totaling 187 amino acids. It includes a 10 a.a N-terminal His tag and has a calculated molecular mass of 21.1 kDa.
Physical Appearance
White, lyophilized powder after filtration.
Formulation
The C19ORF80 protein underwent filtration (0.4 µm) and lyophilization from a 0.5 mg/ml solution in 0.05 M Acetate buffer with a pH of 4.0.
Solubility
To prepare a working stock solution of around 0.5 mg/ml, it is recommended to reconstitute the lyophilized pellet in 0.1 M Acetate buffer (pH 4.0) and allow it to dissolve completely. For adjusting to a higher pH, dilute the solution extensively with an appropriate buffer to a concentration of 10 µg/ml. Note that the solubility of this antigen is limited at higher concentrations. This product is not sterile; therefore, it is essential to filter it through a suitable sterile filter before using it in cell culture.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to avoid repeated freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited time, showing no significant changes after two weeks at this temperature.
Purity
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Betatrophin, Angiopoietin-like protein 8, Lipasin, C19orf80, Angptl8, RIFL, TD26, PRO1185, PVPA599.
Amino Acid Sequence
MKHHHHHHASAPMGGPELAQ HEELTLLFHG TLQLGQALNG VYRTTEGRLT KARNSLGLYG RTIELLGQEV SRGRDAAQEL RASLLETQME EDILQLQAEA TAEVLGEVAQ AQKVLRDSVQ RLEVQLRSAW LGPAYREFEV LKAHADKQSH ILWALTGHVQ RQRREMVAQQ HRLRQIQERL HTAALPA.
