C14ORF129 Human

Chromosome 14 Open Reading Frame 129 Human Recombinant
Cat. No.
BT9238
Source
Escherichia Coli.
Synonyms

GSK3-beta interaction protein, GSKIP, C14orf129, HSPC210, MGC4945.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

C14ORF129 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids (1-139 a.a.) and having a molecular mass of 17.8kDa.
The C14ORF129 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
GSK3-beta interaction protein (C14orf129) is a protein that naturally occurs in the body. It shares similarities with the GSK3beta interaction domain found in Axin. This protein can hinder the activity of GSK3beta within the Wnt signaling pathway. C14orf129 might also influence the impact of GSK3? on neurite outgrowth by suppressing the specific phosphorylation of tau at the serine residue 396.
Description
C14ORF129 Human Recombinant is a single, non-glycosylated polypeptide chain with 159 amino acids (specifically, amino acids 1 to 139). It has a molecular weight of 17.8kDa. This recombinant protein is produced in E. Coli and is fused with a 20 amino acid His tag at its N-terminus. The purification process involves proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The C14ORF129 solution is provided at a concentration of 1 mg/ml. It is buffered with 20mM Tris-HCl at a pH of 8.0. Additionally, it contains 1mM DTT, 10% glycerol, and 50mM NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at a temperature of 4 degrees Celsius. For extended storage, it is recommended to freeze the product at -20 degrees Celsius. Adding a carrier protein, such as HSA or BSA, at a concentration of 0.1% is advisable for long-term storage. It is important to avoid repeatedly freezing and thawing the product.
Purity
The purity of the product is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms

GSK3-beta interaction protein, GSKIP, C14orf129, HSPC210, MGC4945.

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS.

Product Science Overview

Gene and Protein Structure

The C14orf129 gene is one of many open reading frames (ORFs) on chromosome 14. An open reading frame is a continuous stretch of codons that begins with a start codon (usually AUG) and ends at a stop codon (UAA, UAG, or UGA). The protein encoded by C14orf129 is composed of a specific sequence of amino acids, which determines its structure and function.

Biological Significance

While the exact role of the C14orf129 protein is not fully understood, it is believed to be involved in various cellular processes. Proteins encoded by ORFs can play roles in cell signaling, structural integrity, and metabolic pathways. Research is ongoing to determine the specific functions and interactions of the C14orf129 protein within the cell.

Recombinant Protein Production

The production of human recombinant C14orf129 involves cloning the gene into an expression vector, which is then introduced into a host cell (such as bacteria, yeast, or mammalian cells). The host cells are cultured under conditions that promote the expression of the recombinant protein, which is then purified for research or therapeutic use.

Applications in Research

Recombinant proteins like C14orf129 are valuable tools in biomedical research. They can be used to study protein function, interactions, and pathways. Additionally, recombinant proteins are used in drug development, as they can serve as targets for new therapies or as therapeutic agents themselves.

Future Directions

As research continues, the understanding of C14orf129 and its protein product will likely expand. This could lead to new insights into its role in health and disease, and potentially to the development of novel therapeutic strategies.

For more detailed information, you can refer to resources like RayBiotech .

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