BTF3L4 Human

Basic Transcription Factor 3-Like 4 Human Recombinant
Cat. No.
BT26101
Source
Escherichia Coli.
Synonyms
Basic transcription factor 3-like 4, BTF3L4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

BTF3L4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (1-78 a.a) and having a molecular mass of 11.3kDa.
BTF3L4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BTF3L4, a member of the NAC-beta family, plays a crucial role in transcription initiation. It contains a NAC-A/B domain and is essential for this process. This protein binds to newly synthesized polypeptide chains as they emerge from ribosomes, preventing their interaction with the SRP, which typically directs nascent secretory peptides to the endoplasmic reticulum (ER). BTF3L4 also functions as a general transcription factor by forming a stable complex with RNA polymerase II.
Description
Recombinant human BTF3L4, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 101 amino acids (amino acids 1-78). It has a molecular mass of 11.3 kDa. The protein consists of BTF3L4 fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered solution that is colorless.
Formulation
The BTF3L4 protein solution is provided at a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4 M urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
Basic transcription factor 3-like 4, BTF3L4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNQEKLA KLQAQVRIGG KGTARRKKKV VHRTATADDK KLQSSLKKLA VNNIAGIEES WTVKHQNQKT LMRKMMMFQI L.

Product Science Overview

Gene Information
  • Gene Name: Basic Transcription Factor 3-Like 4
  • Gene Symbol: BTF3L4
  • Aliases: MGC23908, Transcription Factor BTF3 Homolog 4
  • Protein Class: Protein-coding
Protein Expression and Localization

BTF3L4 is expressed in various tissues, with general cytoplasmic expression. It is localized mainly to the nucleoplasm and cytosol . The protein evidence is at the protein level, indicating that the presence of this protein has been confirmed through experimental data .

Function

The primary function of BTF3L4 is to assist in the initiation of transcription by binding to emerging polypeptide chains and preventing their premature interaction with the SRP . This function is critical for ensuring that nascent peptides are correctly processed and targeted within the cell.

Tissue Specificity

The RNA expression of BTF3L4 shows low tissue specificity, indicating that it is expressed across a wide range of tissues without significant enrichment in any particular tissue .

Interactions

BTF3L4 interacts with several other proteins, which are essential for its role in transcription initiation and protein processing .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.