Introduction
BTF3L4, a member of the NAC-beta family, plays a crucial role in transcription initiation. It contains a NAC-A/B domain and is essential for this process. This protein binds to newly synthesized polypeptide chains as they emerge from ribosomes, preventing their interaction with the SRP, which typically directs nascent secretory peptides to the endoplasmic reticulum (ER). BTF3L4 also functions as a general transcription factor by forming a stable complex with RNA polymerase II.
Description
Recombinant human BTF3L4, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 101 amino acids (amino acids 1-78). It has a molecular mass of 11.3 kDa. The protein consists of BTF3L4 fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered solution that is colorless.
Formulation
The BTF3L4 protein solution is provided at a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4 M urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
Basic transcription factor 3-like 4, BTF3L4.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNQEKLA KLQAQVRIGG KGTARRKKKV VHRTATADDK KLQSSLKKLA VNNIAGIEES WTVKHQNQKT LMRKMMMFQI L.