BTF3 Human
Description
BTF3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
BTF3 is encoded by the BTF3 gene, which is located on chromosome 5 in humans . The protein consists of multiple domains that facilitate its interaction with RNA polymerase II and other transcription factors. BTF3 is required for the proper assembly of the transcription pre-initiation complex, which is necessary for the accurate initiation of transcription .
The recombinant form of BTF3, often tagged with a His-tag for purification purposes, is expressed in Escherichia coli and purified using conventional chromatography techniques . This recombinant protein retains the functional properties of the native protein, making it a valuable tool for research and biochemical studies.
BTF3 is involved in several critical cellular processes:
- Transcription Initiation: BTF3 is essential for the initiation of transcription by RNA polymerase II. It helps in the formation of the transcription pre-initiation complex, ensuring that transcription starts accurately and efficiently .
- Protein Targeting: When associated with nascent polypeptide-associated complex alpha (NACA), BTF3 prevents the inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). This function is crucial for maintaining cellular homeostasis and protein quality control .
- Gene Regulation: BTF3 is involved in the regulation of various genes, influencing cellular processes such as growth, differentiation, and response to environmental stimuli .
Mutations or dysregulation of the BTF3 gene have been associated with several diseases, including multiple mitochondrial dysfunctions syndrome 5 and polycystic kidney disease . Understanding the role of BTF3 in these conditions can provide insights into their pathogenesis and potential therapeutic targets.
Recombinant BTF3 is widely used in research to study transcription mechanisms, protein-protein interactions, and gene regulation. It serves as a model to understand the complex processes involved in transcription initiation and the role of transcription factors in gene expression.
