BIRC7 Human
Greater than 90.0% as determined by SDS-PAGE.
Description
BIRC7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (1-298 a.a.) and having a molecular mass of 34kDa. BIRC7 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Belonging to the inhibitor of apoptosis protein (IAP) family, BIRC7 possesses a single copy of a baculovirus IAP repeat (BIR) and a RING-type zinc finger domain. The BIR domain, essential for inhibitory activity, interacts with caspases. The RING finger domain, while sometimes enhancing anti-apoptotic activity, doesn't independently inhibit apoptosis. This gene has two identified transcript variants that encode distinct isoforms. These isoforms exhibit different antiapoptotic properties: isoform a protects cells from staurosporine-induced apoptosis, while isoform b provides protection against apoptosis. BIRC7 directly interacts with several caspases, including caspase-3, caspase-7, and caspase-9. It inhibits caspase-9 activation induced by Apaf-1, cytochrome c, and dATP.
Recombinant Human BIRC7, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 318 amino acids (residues 1-298). It has a molecular weight of 34kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
BIRC7 protein solution at a concentration of 1mg/ml in a buffer consisting of 20mM Tris pH 8, 1mM DTT, 0.1M NaCl, 2mM EDTA, and 20% glycerol.
For optimal storage, the product should be kept at 4°C if it will be used within 2-4 weeks. For extended storage, freezing at -20°C is recommended.
To ensure long-term stability during storage, adding a carrier protein such as HSA or BSA (0.1%) is advisable.
Repeated freezing and thawing of the product should be avoided.
The purity of the product is determined to be greater than 90.0% using SDS-PAGE analysis.
MGSSHHHHHH SSGLVPRGSH MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELRLASFYDWPLT AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS.
Product Science Overview
BIRC7 contains a single baculoviral IAP repeat (BIR) domain and a RING-type zinc finger domain . The BIR domain is essential for its inhibitory activity, as it interacts with caspases, a family of protease enzymes that play essential roles in apoptosis . The RING finger domain can enhance the anti-apoptotic activity of BIRC7 but does not inhibit apoptosis on its own .
The primary function of BIRC7 is to inhibit apoptosis by interfering with the proteolytic activation of caspases . This inhibition is crucial for cell survival and proliferation. Overexpression of BIRC7 has been observed in various cancers, including lung, colon, and prostate cancers, suggesting its role in cancer progression and chemotherapy sensitivity .
Recombinant BIRC7 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 304 amino acids . The recombinant protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques . The molecular mass of the recombinant BIRC7 is approximately 33.4 kDa .
