BIRC5 Human

Baculoviral IAP Repeat-Containing 5 Human Recombinant
Cat. No.
BT25583
Source
Escherichia Coli.
Synonyms
BIRC-5, Baculoviral IAP repeat-containing protein 5, API4, EPR-1, Apoptosis inhibitor survivin, Apoptosis inhibitor 4, BIRC5, IAP4, Survivin.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Survivin Human Recombinant fused to a 152 a.a. N-terminal CaM-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294 amino acids (1-142 a.a.) and having a molecular mass of 33 kDa.

Product Specs

Introduction
As a member of the inhibitor of apoptosis (IAP) family, Survivin negatively regulates programmed cell death by inhibiting caspase activation. This protein is characterized by a single BIR domain, unlike other IAP family members that possess multiple BIR domains. Survivin is implicated in various cancers, including Merkel cell carcinoma, lung cancer, and breast carcinomas. Its overexpression is linked to tumor development, progression, and resistance to therapy. For instance, Survivin is upregulated in lung cancer cells compared to normal lung tissue and is associated with poor prognosis. Similarly, Survivin expression is elevated in breast carcinomas, and its upregulation is linked to increased tumor growth and metastasis. Furthermore, Survivin plays a role in suppressing the apoptotic response of intestinal epithelial cells infected with parasites like C. parvum, thereby facilitating parasite survival. Interestingly, Survivin has shown potential as a therapeutic target. Studies have demonstrated that inhibiting Survivin can enhance the effectiveness of oncolytic adenoviruses in treating cancer.
Description
Recombinant human Survivin, fused to a 152 amino acid N-terminal CaM-Tag, is produced in E. coli. This non-glycosylated polypeptide comprises 294 amino acids, with the Survivin sequence spanning amino acids 1 to 142. The resulting protein has a molecular weight of 33 kDa.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The BIRC5 protein is supplied in a solution containing 20mM Tris-HCl buffer at pH 7.5 and 0.1M NaCl.
Stability
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. To ensure stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
BIRC-5, Baculoviral IAP repeat-containing protein 5, API4, EPR-1, Apoptosis inhibitor survivin, Apoptosis inhibitor 4, BIRC5, IAP4, Survivin.
Source
Escherichia Coli.
Amino Acid Sequence
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SHMGAPTLPP AWQPFLKDHR ISTFKNWPFL EGCACTPERM AEAGFIHCPT ENEPDLAQCF FCFKELEGWE PDDDPIEEHK KHSSGCAFLS VKKQFEELTL GEFLKLDRER AKNKIAKETN NKKKEFEETA KKVRRAIEQL AAMD.

Product Science Overview

Gene and Protein Structure

The BIRC5 gene is located on chromosome 17 (17q25.3) and encodes a protein that contains a single baculovirus IAP repeat (BIR) domain . Unlike other IAP family members, BIRC5 lacks a C-terminus RING finger domain . The protein is approximately 16.5 kDa in size and is composed of 142 amino acids .

Function and Mechanism

Survivin is a multitasking protein with dual roles in promoting cell proliferation and preventing apoptosis . It is a component of the chromosome passage protein complex (CPC), which is essential for chromosome alignment and segregation during mitosis and cytokinesis . Survivin directs CPC movement to different locations from the inner centromere during prometaphase to the midbody during cytokinesis .

The protein functions by inhibiting caspase activation, thereby leading to the negative regulation of apoptosis . This inhibition is crucial for cell survival, especially in proliferating cells and during fetal development .

Expression and Clinical Significance

Survivin is highly expressed during fetal development and in most human tumors, but its expression is low in adult tissues . This differential expression pattern makes it a potential target for cancer therapy, as it can discriminate between transformed (cancerous) and normal cells .

The overexpression of Survivin in various cancers, including adenocarcinoma and cervical intraepithelial neoplasia, has been associated with poor prognosis . Its role in inhibiting apoptosis and promoting cell proliferation makes it a key player in tumorigenesis .

Recombinant Production

Human recombinant BIRC5 can be produced using various expression systems, including bacterial, yeast, and mammalian cells. The recombinant protein is often used in research to study its function, mechanism, and potential as a therapeutic target.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

BIRC7 Human
Baculoviral IAP Repeat-Containing 7 Human Recombinant
Cat. No.
BT25679
Source
Escherichia Coli.
BIRC7 Human, (1-280 a.a.)
Baculoviral IAP Repeat-Containing 7 (1-280 a.a.) Human Recombinant
Cat. No.
BT25776
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.