BTC Human

Human Betacellulin is encoded by the BTC gene located on chromosome 4 . The mature form of BTC is a heparin-binding protein composed of 80 amino acid residues . The recombinant human Betacellulin protein is typically produced in E. coli and has a molecular weight of approximately 9.9 kDa . The protein is highly pure, with a purity greater than 97% as determined by SDS-PAGE .

Betacellulin is known for its ability to bind to and activate the ErbB family of receptor tyrosine kinases, including EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4 . This interaction results in the activation of downstream signaling pathways such as the PI3K and Erk pathways, which are crucial for cell proliferation and survival .

Betacellulin has been extensively studied for its role in the proliferation of pancreatic beta cell lines in vitro . Research has shown that BTC can induce beta cell regeneration and increase insulin secretion in rodent models of diabetes . This makes it a promising candidate for therapeutic applications in diabetes treatment.

Recombinant human Betacellulin is produced in a carrier-free form, which means it does not contain Bovine Serum Albumin (BSA) as a carrier protein . This enhances the protein’s stability and shelf-life, making it suitable for various applications, including cell or tissue culture and ELISA standards . The protein is typically lyophilized from a filtered solution in PBS with Trehalose and can be reconstituted at 100-500 μg/mL in PBS . It is stable for up to 12 months when stored at -20 to -70°C .