BTC Bovine

Betacellulin Bovine Recombinant
Cat. No.
BT15700
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Betacellulin Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton.
Betacellulin Bovine Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Betacellulin (BTC) is a strong stimulator of cell division for retinal pigment epithelial cells and vascular smooth muscle cells. Its effects are likely due to its interaction with the epidermal growth factor receptor (EGFR) and similar receptors.
Description
Recombinant Bovine Betacellulin, produced in E. coli, is a single-chain polypeptide with 80 amino acids. It is non-glycosylated and has a molecular weight of 9003 Daltons. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
Following extensive dialysis against 50mM acetic acid, the Recombinant Bovine Betacellulin is lyophilized.
Solubility
The lyophilized BTC Bovine should be reconstituted in sterile 18 megaohm-cm H2O to a concentration of at least 100 micrograms per milliliter. This solution can then be diluted further into other aqueous solutions.
Stability
While Lyophilized Betacellulin Bovine Recombinant is stable at room temperature for up to 3 weeks, it's recommended to store it desiccated below -18 degrees Celsius. After reconstitution, store BTC Bovine at 4 degrees Celsius for 2-7 days. For long-term storage, freeze below -18 degrees Celsius. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 95.0% as determined by:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity

The effective dose that results in 50% of the maximum response (ED50) is less than 10.0 nanograms per milliliter. This value was determined by the dose-dependent proliferation of murine BALB/C 3T3 cells as measured through 3H-thymidine uptake. This corresponds to a specific activity of 100,000 units per milligram.

Protein Content
Two independent methods were used to quantify protein content:
1. UV spectroscopy at 280 nanometers, utilizing an absorbance value of 0.59 as the extinction coefficient for a 0.1% (1 milligram per milliliter) solution. This value is calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, with a calibrated solution of BTC serving as a reference standard.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr.

Product Science Overview

Structure and Function

Betacellulin is synthesized as a transmembrane precursor and is then proteolytically cleaved to release the mature, soluble form of the protein . The mature form of BTC is a heparin-binding protein that plays a crucial role in cell proliferation, differentiation, and survival . It is known to interact with the EGF receptor (EGFR) and ErbB4, leading to the activation of downstream signaling pathways that regulate these cellular processes .

Recombinant Betacellulin

Recombinant Betacellulin, including the bovine version, is produced using various expression systems such as E. coli or HEK293 cells . The recombinant protein is often used in research to study its biological activities and potential therapeutic applications. For instance, recombinant human Betacellulin has been shown to promote the proliferation of Balb/3T3 mouse embryonic fibroblast cells .

Applications

Recombinant Betacellulin is utilized in various research areas, including:

  • Cell Culture: As a supplement to promote cell growth and proliferation.
  • Signal Transduction Studies: To investigate the activation of EGFR and ErbB4 signaling pathways.
  • Therapeutic Research: Exploring its potential in regenerative medicine and cancer therapy.
Storage and Stability

Recombinant Betacellulin is typically lyophilized from a filtered solution and can be reconstituted in phosphate-buffered saline (PBS) for use . It is recommended to store the protein at -20 to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .

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