BD 1 Human
(b) Analysis by SDS-PAGE.
Description
The BD-1 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Beta Defensin-1 is encoded by the DEFB1 gene in humans . The gene is located on chromosome 8 and is closely related to other defensin family members, such as alpha defensins . The protein itself is composed of a sequence that includes a six-cysteine motif, which forms three intra-molecular disulfide bonds. These bonds are crucial for the protein’s stability and function .
BD-1 is primarily expressed by epithelial cells, which line the surfaces and cavities of the body, including the skin, respiratory tract, and gastrointestinal tract . The expression of BD-1 is constitutive, meaning it is continuously produced at a baseline level, unlike some other defensins that are induced in response to infection or inflammation .
The primary function of BD-1 is to act as an antimicrobial peptide. It disrupts the membrane integrity of microbes, leading to their death. This activity is essential for the resistance of epithelial surfaces to microbial colonization . Additionally, BD-1 has been implicated in the pathogenesis of certain diseases, such as cystic fibrosis, where its antimicrobial activity is compromised .
Recombinant human Beta Defensin-1 is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is typically expressed as a fusion protein, which includes a tag to facilitate purification. After expression, the protein is purified using techniques such as affinity chromatography, and the tag is often removed to yield the mature protein .
The recombinant form of BD-1 retains its antimicrobial properties and is used in various research applications to study its function and potential therapeutic uses .
BD-1 has several potential applications in both clinical and research settings. In clinical research, it is studied for its role in various diseases, including cystic fibrosis, asthma, and sepsis . Understanding the function and regulation of BD-1 can provide insights into the development of new therapeutic strategies for these conditions.
In addition to its antimicrobial properties, BD-1 is also being investigated for its role in modulating the immune response. It has been shown to interact with various components of the immune system, potentially influencing inflammation and immune cell recruitment .
